Kopchaliiska D, Stamenova M, Manolova V, Kehayov I, Dakovska L, Kyurkchiev S
Department of Molecular Immunology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Clin Immunol Immunopathol. 1998 May;87(2):130-8. doi: 10.1006/clin.1997.4509.
Investigations on the specific idiotypes of autoantibodies are supposed to help with the understanding of the control mechanisms participating in the pathogenesis of autoimmune diseases. This study describes three monoclonal antibodies (Mabs) that recognize distinct idiotypic determinants on anti-insulin autoantibodies. The preabsorption by IAA-positive sera of insulin inhibits their subsequent binding to the anti-Id, thus suggesting that the Mabs recognize epitopes located at or near the binding site of insulin autoantibodies (IAA). These idiotypes are detected in sera from patients with insulin-dependent diabetes mellitus (IDDM), which are IAA-negative, also. It is possible that the expression of the idiotypes recognized might generally be associated with induction of autoantibodies, since they were found in sera from patients with rheumatoid arthritis (RA), autoimmune thyroid disease (AITD), and cataract (K). It can be assumed that the corresponding idiotypes of these Mabs, or similar structures (sequential or conformational), are expressed on autoantibodies with various antigen-binding specificities. These data suggest that some autoimmune diseases are preceded by the secretion of autoantibodies which express a common or similar pathological idiotype.
对自身抗体特异性独特型的研究旨在帮助理解参与自身免疫性疾病发病机制的调控机制。本研究描述了三种单克隆抗体（Mabs），它们识别抗胰岛素自身抗体上不同的独特型决定簇。胰岛素的IAA阳性血清预吸收可抑制其随后与抗独特型抗体的结合，因此表明这些单克隆抗体识别位于胰岛素自身抗体（IAA）结合位点或其附近的表位。这些独特型在胰岛素依赖型糖尿病（IDDM）患者的血清中也能检测到，这些患者的血清也是IAA阴性的。由于在类风湿性关节炎（RA）、自身免疫性甲状腺疾病（AITD）和白内障（K）患者的血清中也发现了这些独特型，因此有可能所识别的独特型的表达通常与自身抗体的诱导有关。可以假定这些单克隆抗体的相应独特型或类似结构（序列或构象）在具有各种抗原结合特异性的自身抗体上表达。这些数据表明，一些自身免疫性疾病之前会分泌表达共同或相似病理独特型的自身抗体。
