Buroker N, Day J, Maclaren D, Sweeny K, Scott C R, Chen S H
Department of Pediatrics, School of Medicine, University of Washington, Seattle, USA.
Gene Geogr. 1997 Apr;11(1):1-14.
We studied four variable number of tandem repeat (VNTR) loci (D4S139, D10S28, D17S74, and D17S79) in five ethnic populations from the Seattle metropolitan area. DNA samples purified from randomly chosen individuals were digested with Hae III or Hinf I and probed with pH30, for D4S139; TBQ7 for D10S28; pCMM86 for D17S74 and pAC256 for D17S79. The allele frequencies, expected Hardy-Weinberg values, observed heterozygosities and genetic distances among the populations were obtained for all these loci. D4S139 restriction fragment lengths (RFLs) varied in size from 1.4 to 22 kilobase pairs (kbp). The observed heterozygosities (H) varied from 84% in Native American populations to 94% among Blacks. D10S28 RFLs varied in size from 650 base pairs (bp) to 10.1 kbp. H varied from 90% in Native Americans to 96% in Caucasians and Hispanics. D17S74 RFLs varied in size from 782 bp to 9.3 kbp. H varied from 87% in Asians to 92% among Blacks. D17S79 RFLs varied in size from 400 bp to 3 kbp. H varied from 87% in Hispanics to 95% in the Black population. The frequencies of genotypes of the loci conformed to Hardy-Weinberg equilibrium with the exception of the D17S79 in Hispanics and Native Americans. The genetic distances between the populations were also determined.
我们研究了西雅图市区五个种族群体中的四个串联重复可变数目(VNTR)基因座(D4S139、D10S28、D17S74和D17S79)。从随机选取的个体中纯化的DNA样本用Hae III或Hinf I进行消化，并用pH30探测D4S139；用TBQ7探测D10S28；用pCMM86探测D17S74，用pAC256探测D17S79。获得了所有这些基因座的等位基因频率、预期的哈迪-温伯格值、观察到的杂合度以及群体间的遗传距离。D4S139限制性片段长度(RFL)大小从1.4千碱基对(kbp)到22千碱基对不等。观察到的杂合度(H)在美洲原住民群体中为84%，在黑人中为94%。D10S28 RFL大小从650碱基对(bp)到10.1千碱基对不等。H在美洲原住民中为90%，在白人和西班牙裔中为96%。D17S74 RFL大小从782碱基对到9.3千碱基对不等。H在亚洲人中为87%，在黑人中为92%。D17S79 RFL大小从400碱基对到3千碱基对不等。H在西班牙裔中为87%，在黑人中为95%。除了西班牙裔和美洲原住民中的D17S79外，这些基因座的基因型频率符合哈迪-温伯格平衡。还确定了群体之间的遗传距离。
