Grossi G F, Durante M, Gialanella G, Pugliese M, Mosse I
Dipartimento di Scienze Fisiche, Università di Napoli Federico II, Italy.
Radiat Environ Biophys. 1998 Apr;37(1):63-7. doi: 10.1007/s004110050094.
The search for effective radioprotectors is of major concern in the medical, military, environmental, and space sciences. Conventional radioprotectors are generally effective only during a single irradiation and display their radioprotective properties only at high, toxic concentrations. In addition, they reduce somatic radiation effects but are poorly efficient in protecting from hereditary stochastic radiation effects. In this respect, the pigment melanin merits attention. Experiments referring to potential melanin effects on the ionising radiation response have been carried out with different biological systems, both in vivo and in vitro. In this paper, we present results on the response to high- and low-linear energy transfer (LET) radiation of a human mammary epithelial cell line, H184B5 F5-1 M/10, supplemented by melanin. The incorporation of auto-oxidative (L-dopa) melanin was linear for concentrations from 3 to 10 micrograms/ml in the growth medium. Concentrations of up to 250 micrograms/ml did not significantly impair the cells proliferative ability. No significant protective effect of melanin on the survival of cultured cells after exposure to alpha-particles (130 keV/micron) or x-rays was observed.
寻找有效的辐射防护剂是医学、军事、环境和空间科学领域的主要关注点。传统的辐射防护剂通常仅在单次照射期间有效，并且仅在高毒性浓度下才显示出其辐射防护特性。此外，它们可减轻躯体辐射效应，但在预防遗传性随机辐射效应方面效率低下。在这方面，色素黑色素值得关注。针对黑色素对电离辐射反应的潜在影响，已经在不同的生物系统中进行了体内和体外实验。在本文中，我们展示了人乳腺上皮细胞系H184B5 F5-1 M/10在添加黑色素后对高、低线性能量传递（LET）辐射的反应结果。在生长培养基中，自氧化（L-多巴）黑色素的掺入量在3至10微克/毫升的浓度范围内呈线性。高达250微克/毫升的浓度并未显著损害细胞的增殖能力。未观察到黑色素对暴露于α粒子（130 keV/微米）或X射线后的培养细胞存活有显著的保护作用。
