Tasaki K, Tagawa M, Gunji Y, Matsubara H, Takenaga K, Muramatsu M, Fujimura S, Suzuki T, Asano T, Ochiai T, Isono K, Kouzu T, Sakiyama S
Department of Surgery (II), School of Medicine, Chiba University, Japan.
Anticancer Res. 1998 Mar-Apr;18(2A):813-7.
We examined the antitumor effect of low and high interleukin-2 (IL-2) producers of murine colon carcinoma cells (Colon 26) which were generated by transduction with IL-2 gene in an experimental lung metastasis model using syngeneic mice. Intravenous injection of the low IL-2 producer cells formed multiple lung metastatic foci and the survival of the mice was not different from that of the mice injected with wild-type cells. However, the mice administrated with the high producer cells survived significantly longer. Subcutaneous inoculation of the low producers, although it caused the development of local tumors at the inoculation sites in some of the mice tested, inhibited lung metastasis of wild-type cells subsequently inoculated and prolonged the survival of the mice rechallenged with Meth A cells, syngeneic fibrosarcoma cells. In contrast, inoculation of the high producers did not cause the development of subcutaneous tumors and inhibited the experimental metastasis of parental but not Meth A cells inoculated thereafter. Thus, the amount of secreted IL-2 from tumor cells differentially influences antitumor effects by inducing tumor specific and nonspecific immunity.
我们在同基因小鼠实验性肺转移模型中，研究了通过白细胞介素-2（IL-2）基因转导产生的低IL-2分泌型和高IL-2分泌型小鼠结肠癌细胞（Colon 26）的抗肿瘤作用。静脉注射低IL-2分泌型细胞形成了多个肺转移灶，这些小鼠的生存率与注射野生型细胞的小鼠没有差异。然而，给予高分泌型细胞的小鼠存活时间显著延长。皮下接种低分泌型细胞，尽管在一些受试小鼠的接种部位引发了局部肿瘤的生长，但随后抑制了野生型细胞的肺转移，并延长了再次接种同基因纤维肉瘤细胞Meth A的小鼠的存活时间。相比之下，接种高分泌型细胞不会导致皮下肿瘤的生长，且抑制了亲本细胞而非随后接种的Meth A细胞的实验性转移。因此，肿瘤细胞分泌的IL-2量通过诱导肿瘤特异性和非特异性免疫，对抗肿瘤作用产生不同影响。
