Gebauer G, Jäger W, Lang N
Department of Obstetrics and Gynaecology, University of Erlangen-Nuremberg, Erlangen, Germany.
Anticancer Res. 1998 Mar-Apr;18(2A):1191-5.
IGF-1 and 2 are thought to be important growth factors for breast cancer. However, gene expression of IGFs or IGF receptors in breast cancer tissues, and especially in metastatic breast cancer cells, is not well known. Expression of mRNA encoding for IGF-1, IGF-2, IGF-receptor 1 and 2, IGF binding proteins- 1 to -6, insulin receptor and insulin was determined in the NIH MCF-7 breast cancer cell line, in specimens from breast cancer tissues, and in 6 primary breast cancer cell cultures obtained from metastatic breast cancer, using rT-PCR technique. Specific mRNA sequences encoding for IGF-receptor 1 and 2, IGFBP-2, -4 and insulin receptor were identified in all cell cultures and most of the tissue specimens. Though in most of the tissues additional expression of IGF-1 and IGF-2 was detected, there was no mRNA encoding for these proteins in MCF-7 cell cultures as well as in the primary cell cultures of metastatic breast cancers. In none of our specimens mRNA encoding for IGFBP-1, -3, -5, -6 and insulin was detectable. IGF-receptor expression in cancer tissues and metastatic breast cancer cells supports the hypothesis that IGFs increase tumor cell proliferation in vivo. Expression of IGF-1 and IGF-2 in tumor tissues but not in cancer cell cultures indicates an IGF expression located predominantly in stromal parts of cancer tissues.
胰岛素样生长因子-1（IGF-1）和胰岛素样生长因子-2（IGF-2）被认为是乳腺癌重要的生长因子。然而，IGF及其受体在乳腺癌组织尤其是转移性乳腺癌细胞中的基因表达情况尚不明确。采用逆转录聚合酶链反应（rT-PCR）技术，对美国国立卫生研究院（NIH）的MCF-7乳腺癌细胞系、乳腺癌组织标本以及取自转移性乳腺癌的6种原发性乳腺癌细胞培养物中编码IGF-1、IGF-2、IGF受体1和2、IGF结合蛋白-1至-6、胰岛素受体和胰岛素的mRNA表达进行了测定。在所有细胞培养物和大多数组织标本中均鉴定出了编码IGF受体1和2、IGFBP-2、-4以及胰岛素受体的特异性mRNA序列。尽管在大多数组织中检测到了IGF-1和IGF-2的额外表达，但在MCF-7细胞培养物以及转移性乳腺癌的原代细胞培养物中均未检测到编码这些蛋白的mRNA。在我们所有的标本中均未检测到编码IGFBP-1、-3、-5、-6和胰岛素的mRNA。癌症组织和转移性乳腺癌细胞中IGF受体的表达支持了IGF在体内增加肿瘤细胞增殖的假说。肿瘤组织中IGF-1和IGF-2的表达而非癌细胞培养物中的表达表明，IGF表达主要位于癌组织的基质部分。
