Quesada C F, Kimata H, Mori M, Nishimura M, Tsuneyoshi T, Baba S
Second Department of Surgery, Hamamatsu University School of Medicine, Shizuoka.
Jpn J Cancer Res. 1998 Apr;89(4):392-6. doi: 10.1111/j.1349-7006.1998.tb00576.x.
The use of nonsteroidal anti-inflammatory drugs has been suggested to have a chemopreventive effect against colon carcinoma, through the inhibition of cyclooxygenases 1 and 2, in patients with familial adenomatous polyposis and in animal models. Acarbose, an alpha-glycosidase inhibitor, may also be chemopreventive. In order to examine the effects of these drugs we employed APC gene knockout mice randomized into 3 groups, one for treatment with piroxicam (0.05% concentration in drinking water), one for acarbose (0.04% concentration in food) and another for the control. After 14 weeks of treatment, mice were killed for quantitation of gastric and intestinal adenomas. Tumor multiplicity in the whole gastrointestinal tract decreased from 33.89 +/- 13.07 tumors/mouse in the control group to 17.05 +/- 7 tumors/mouse in the piroxicam-treated group (P < 0.001). The decrease in the acarbose-treated group (29.68 +/- 12.86 tumors/mouse) was not significant (P < 0.05). The number of tumors > or = 3 mm in diameter was also quantified in all gastrointestinal segments. The number of such tumors in the piroxicam group was decreased to 0.56 +/- 1.2 tumors/mouse from the control value of 3.78 +/- 1.17 tumors/mouse (P < 0.001), while in the acarbose-treated group the number decreased to 2.36 +/- 1.7 tumors/mouse (P < 0.01). Thus, piroxicam decreases the size and number of gastrointestinal adenomas in APC 1309 knockout mice, while acarbose decreases only the size.
非甾体抗炎药的使用已被认为通过抑制环氧化酶1和2，对家族性腺瘤性息肉病患者和动物模型中的结肠癌具有化学预防作用。阿卡波糖，一种α-糖苷酶抑制剂，也可能具有化学预防作用。为了研究这些药物的效果，我们将APC基因敲除小鼠随机分为3组，一组用吡罗昔康治疗（饮用水中浓度为0.05%），一组用阿卡波糖治疗（食物中浓度为0.04%），另一组作为对照。治疗14周后，处死小鼠以定量胃和肠腺瘤。整个胃肠道的肿瘤多发性从对照组的33.89±13.07个肿瘤/小鼠降至吡罗昔康治疗组的17.05±7个肿瘤/小鼠（P<0.001）。阿卡波糖治疗组（29.68±12.86个肿瘤/小鼠）的减少不显著（P<0.05）。还对所有胃肠道段中直径≥3mm的肿瘤数量进行了定量。吡罗昔康组中此类肿瘤的数量从对照组的3.78±1.17个肿瘤/小鼠降至0.56±1.2个肿瘤/小鼠（P<0.001），而在阿卡波糖治疗组中，数量降至2.36±1.7个肿瘤/小鼠（P<0.01）。因此，吡罗昔康可减少APC 1309敲除小鼠胃肠道腺瘤的大小和数量，而阿卡波糖仅减少大小。
