Tsuji F, Shirasawa E
Discovery Research Division, Santen Pharmaceutical Co., Ltd., Osaka, Japan.
Curr Eye Res. 1998 May;17(5):501-5. doi: 10.1076/ceyr.17.5.501.5194.
We investigated the role of platelet-activating factor (PAF) in cell infiltration in endotoxin-induced uveitis (EIU) in guinea pigs.
To elicit EIU, lipopolysaccharide (LPS) was injected into the anterior chamber of the eye. Cell numbers in the aqueous humor after LPS injection were determined by flow cytometry. PAF and prostaglandin E2 (PGE2) production after LPS injection were also examined.
Intracameral injection of LPS induced cell infiltration into the anterior chamber, and platelet-activating factor (PAF) was detected in the aqueous humor. In addition, topical apafant (PAF antagonist) partially inhibited cell infiltration. Intracameral injection of PAF scarcely induced cell infiltration but the reaction with EIU was accelerated by intracameral injection of a small amount of PAF.
These results suggest that PAF has weak direct activity on cell infiltration in intraocular inflammation but enhances intraocular inflammation.
我们研究了血小板活化因子（PAF）在豚鼠内毒素诱导性葡萄膜炎（EIU）细胞浸润中的作用。
为诱发EIU，将脂多糖（LPS）注入眼前房。通过流式细胞术测定LPS注射后房水中的细胞数量。还检测了LPS注射后PAF和前列腺素E2（PGE2）的产生。
前房内注射LPS诱导细胞浸润至前房，且在房水中检测到血小板活化因子（PAF）。此外，局部应用阿帕泛（PAF拮抗剂）可部分抑制细胞浸润。前房内注射PAF几乎不诱导细胞浸润，但前房内注射少量PAF可加速与EIU的反应。
这些结果表明，PAF对眼内炎症中的细胞浸润具有较弱的直接活性，但可增强眼内炎症。
