Institóris L, Siroki O, Dési I, Lesznyák J, Serényi P, Szekeres E, Petri I
Department of Public Health, Albert Szent-Györgyi University Medical School, Szeged, Hungary.
Hum Exp Toxicol. 1998 Apr;17(4):206-11. doi: 10.1177/096032719801700402.
To indicate the immunotoxic potential of chemicals the examinations prescribed by OECD Guideline 407 were extended by the following additional toxicological, haematological, histopathological, and immune function examinations: absolute and relative organ weight of spleen, thymus, popliteal lymph nodes, lung and brain; histopathology of thymus, mesenteric lymph nodes, popliteal lymph nodes, bone marrow (femur), Peyer's patches (ileum), lungs and colon; PFC assay (spleen), T cell proliferation and NK cell assay. Two well known immunosuppressants Azathioprine (AZA) and Cyclosporine A (CysA) were chosen as model compounds at a dose range which do not cause visible toxic signs on the animals during a 28 days treatment period. The results show that the applied experimental system is much more sensitive in detection of the immunotoxic potential of these two compounds in a low dose range than the examination required by OECD Guideline 407 are.
为了表明化学物质的免疫毒性潜力，按照经合组织（OECD）第407号指南规定的检查，通过以下额外的毒理学、血液学、组织病理学和免疫功能检查进行了扩展：脾脏、胸腺、腘窝淋巴结、肺和脑的绝对和相对器官重量；胸腺、肠系膜淋巴结、腘窝淋巴结、骨髓（股骨）、派伊尔氏淋巴集结（回肠）、肺和结肠的组织病理学；PFC测定（脾脏）、T细胞增殖和NK细胞测定。选择两种知名的免疫抑制剂硫唑嘌呤（AZA）和环孢素A（CysA）作为模型化合物，其剂量范围在28天的治疗期内不会对动物造成明显的毒性迹象。结果表明，所应用的实验系统在检测这两种化合物在低剂量范围内的免疫毒性潜力方面比经合组织第407号指南要求的检查更为敏感。
