Tian Z G, Woody M A, Sun R, Welniak L A, Raziuddin A, Funakoshi S, Tsarfaty G, Longo D L, Murphy W J
IRSP, SAIC-Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
Stem Cells. 1998;16(3):193-9. doi: 10.1002/stem.160193.
Recombinant human growth hormone (rhGH) was administered to mice after syngeneic bone marrow transplantation (BMT) to determine its effect on hematopoietic reconstitution. BALB/c mice were given 10 microg intraperitoneal injections of rhGH every other day for a total of 10 injections following syngeneic BMT. Mice that received rhGH exhibited significant increases in total hematopoietic progenitor cell content (colony-forming unit-culture) in both bone marrow and spleen. Erythroid cell progenitor content (burst-forming unit-erythroid) was also significantly increased after rhGH treatment. Analysis of peripheral blood indicated that administration of rhGH resulted in significant increases in the rate of white blood cell and platelet recovery. Granulocyte marker 8C5+ cells were also increased in the bone marrow and spleens of treated mice. Red blood cell, hematocrit, and hemoglobin levels were increased at all time points after rhGH treatment. No significant pathologic effects or weight gain were observed in mice receiving repeated injections of 10 microg rhGH. Thus, rhGH administration after syngeneic BMT promoted multilineage hematopoietic reconstitution and may be of clinical use for accelerating hematopoiesis after autologous BMT.
在同基因骨髓移植（BMT）后给小鼠注射重组人生长激素（rhGH），以确定其对造血重建的影响。同基因BMT后，给BALB/c小鼠每隔一天腹腔注射10微克rhGH，共注射10次。接受rhGH的小鼠骨髓和脾脏中的总造血祖细胞含量（集落形成单位培养）显著增加。rhGH治疗后，红系细胞祖细胞含量（红系爆式集落形成单位）也显著增加。外周血分析表明，注射rhGH导致白细胞和血小板恢复率显著提高。在接受治疗的小鼠的骨髓和脾脏中，粒细胞标志物8C5+细胞也增加。rhGH治疗后的所有时间点，红细胞、血细胞比容和血红蛋白水平均升高。在接受10微克rhGH重复注射的小鼠中未观察到明显的病理效应或体重增加。因此，同基因BMT后注射rhGH可促进多系造血重建，可能在临床上用于加速自体BMT后的造血。
