Grossman B M, Hobbs J K, Edwards G L, Martin R J
Department of Foods and Nutrition, University of Georgia, Athens 30602, USA.
Physiol Behav. 1998 Mar;63(5):745-50. doi: 10.1016/s0031-9384(97)00535-0.
Our previous work indicates that type and level of dietary fat influences selection of protein and carbohydrate diets. Serotonin (5HT) appears to be involved in this feeding behavior. In the present study, we examined the effects of 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) on feeding behavior, and fenfluramine on in vitro 5HT and 5-hydroxyindoleacetic acid (5HIAA) release from the brain in rats fed tallow or corn oil. Male, Sprague-Dawley rats were given a diet containing corn oil or tallow for 2 days. In Experiment 1, rats received an injection of 8-OH-DPAT (a 5HT1A agonist) or saline and were then allowed to select from two diets: low protein/high carbohydrate or high protein/low carbohydrate. Prior exposure to tallow caused an increased intake of protein and 8-OH-DPAT blunted this effect. In Experiment 2, the dorsal raphe from rats fed tallow or corn oil was superfused with fenfluramine or vehicle. Superfusates were collected for analysis of 5HT and 5HIAA. Fenfluramine increased serotonin release in tallow-fed animals as compared to basal. These results suggest that serotonin may be involved in mediating tallow's effect on macronutrient selection.
我们之前的研究表明，膳食脂肪的类型和水平会影响蛋白质和碳水化合物饮食的选择。血清素（5-羟色胺，5HT）似乎参与了这种摄食行为。在本研究中，我们检测了8-羟基-2-（二正丙基氨基）四氢萘（8-OH-DPAT）对摄食行为的影响，以及芬氟拉明对喂食牛脂或玉米油的大鼠大脑中5HT和5-羟吲哚乙酸（5HIAA）体外释放的影响。将雄性斯普拉格-道利大鼠给予含玉米油或牛脂的饮食2天。在实验1中，给大鼠注射8-OH-DPAT（一种5HT1A激动剂）或生理盐水，然后让它们从两种饮食中选择：低蛋白/高碳水化合物或高蛋白/低碳水化合物。预先接触牛脂会导致蛋白质摄入量增加，而8-OH-DPAT减弱了这种作用。在实验2中，用芬氟拉明或赋形剂对喂食牛脂或玉米油的大鼠的中缝背核进行灌流。收集灌流液以分析5HT和5HIAA。与基础水平相比，芬氟拉明增加了喂食牛脂动物的血清素释放。这些结果表明，血清素可能参与介导牛脂对常量营养素选择的影响。
