Dietary and tissue residue analysis and contaminant intake estimations in rats consuming diets composed of Great Lakes salmon: a multigeneration study.

To further characterize the toxicological risk associated with chemical contaminants in Great Lakes fish, a multigeneration rat reproduction study was designed. Mature chinook salmon (Oncorhynchus tsawytscha), collected during the Fall 1991 spawning runs from Sydenham River, Lake Huron, and Credit River, Lake Ontario, were filleted, lyophilized, and incorporated into standard rat diets at 25% (w/w) or 100% (w/w) of the normal protein compliment [casein, 20% (w/w)]. This resulted in diets composed of 5 or 20% (w/w) lyophilized fish and estimated daily fish intakes by the rats at levels approximately 15- and 60-fold greater, respectively, than the current estimate for the Canadian public for all fish and seafood. Both fresh and lyophilized fish were analyzed for the following groups of contaminants: halogenated aromatic hydrocarbons [polychlorinated biphenyls, dibenzodioxins, and dibenzofurans (PCBs, PCDDs, PCDFs)], polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides, metals, volatile organics, and other extractable organics (chlorinated phenols and benzenes). In general, only minor site differences existed for the specific types of contaminants detected; however, fish from the Credit River contained slightly greater amounts of PCBs (2- to 3-fold), dioxin toxic equivalencies (TCDD TEQs; 1.5- to 2.0-fold), DDT and metabolites (1. 5-fold), and appreciably higher amounts of mirex (15-fold). This general pattern of contaminant differences continued when the various diets were prepared using the lyophilized fish. Tissue samples (adipose, liver) were taken from the animals at various stages of the study and also analyzed for the same groups of contaminants. In general, adipose tissue was the major reservoir for organochlorine (OC) pesticides and PCBs, while "dioxin-like" PCDD/DF congeners and mercury were found preferentially in the liver. Contaminant intake calculations and tissue residue levels are provided.