[3H]血清素释放：一种用于测量肥大细胞脱颗粒的改进方法。

[3H]serotonin release: an improved method to measure mast cell degranulation.

作者信息

Mazingue C, Dessaint J P, Capron A

出版信息

J Immunol Methods. 1978;21(1-2):65-77. doi: 10.1016/0022-1759(78)90224-7.

Abstract

A method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. Mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin B sulphate, ATP, bovine chymotrypsin and L-alpha-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. These two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell degranulation test, and a methodological study of the release of [3H]serotonin is described. The measurement of labelled serotonin release provides a simple and quick assay of mast cell degranulation compared to the time required for the classical rat mast cell degranulation technique and achieves a greater sensitivity.

摘要

本文描述了一种基于啮齿动物中活化肥大细胞释放氚标记血清素的方法。肥大细胞选择性地摄取标记的血清素，并在被非特异性刺激物（化合物48/80、硫酸多粘菌素B、ATP、牛胰凝乳蛋白酶和L-α-溶血磷脂酰胆碱）或过敏抗体及相应抗原激活后释放标记物。与甲苯胺蓝显微镜下大鼠肥大细胞脱颗粒试验相比，对这两种激活类型进行了研究，并描述了[3H]血清素释放的方法学研究。与经典大鼠肥大细胞脱颗粒技术所需时间相比，标记血清素释放的测量提供了一种简单快速的肥大细胞脱颗粒检测方法，且具有更高的灵敏度。