Wu Z, Tong W G
Department of Chemistry, San Diego State University, CA 92182, USA.
J Chromatogr A. 1998 May 1;805(1-2):63-9. doi: 10.1016/s0021-9673(98)00022-3.
Nonlinear optical phase conjugation by degenerate four-wave mixing is demonstrated as a sensitive "absorbance" detection method for microbore high-performance liquid chromatography. An argon ion laser operating at the 488-nm line is used as the excitation light source to generate the wave-mixing signal for dabsyl-labeled amino acids. Advantages of the nonlinear laser detection method include: virtually 100% optical signal collection efficiency, generation of the signal in the form of a coherent laser beam, signal measurement against a virtually dark background, reliable detection of small absorbance values, excellent detection sensitivity for both fluorescing and non-fluorescing analytes, relatively simple one-color one-laser optical setup, and low power or energy requirements for continuous-wave or pulsed lasers. Using our one-laser one-color nonlinear laser detector for "absorbance" measurements in liquid chromatography, we report a crude preliminary "injected" detection limit of 780 fmol for glycine.
简并四波混频产生的非线性光学相位共轭被证明是一种用于微径高效液相色谱的灵敏“吸光度”检测方法。使用工作在488纳米谱线的氩离子激光器作为激发光源，以产生针对丹磺酰氯标记氨基酸的波混频信号。非线性激光检测方法的优点包括：几乎100%的光信号收集效率、以相干激光束形式产生信号、在几乎黑暗的背景下进行信号测量、可靠检测小吸光度值、对荧光和非荧光分析物均具有出色的检测灵敏度、相对简单的单波长单激光器光学装置以及对连续波或脉冲激光器的低功率或能量要求。使用我们的单激光器单波长非线性激光检测器进行液相色谱中的“吸光度”测量，我们报告了甘氨酸的粗略初步“进样”检测限为780飞摩尔。
