Iype LE, Michael M, Verma M, Iype PT
Biological Research Faculty and Facility, Inc., Ijamsville, Maryland 21754, USA.
Cytotechnology. 1998;26(3):207-18. doi: 10.1023/a:1007953429252.
New human breast cell lines were developed from metastatic breast cancer tissues and normal breast tissues. Primary cultures were initiated from cellular outgrowths of explanted tissues or from mechanically isolated cells in two serum-free media. Cell cultures derived from both cancer and normal tissues were immortalized with pRSV-T plasmid to generate permanent breast cell lines that exhibited an epithelial morphology. Cell lines generated in this study were characterized with respect to morphology, growth rate, karyotype, presence of specific genes, and the expression of epithelial and breast markers. The cell lines expressed the epithelial cell markers, cytokeratins 8 and 18, and retained the capacity to produce human milk fat globulin. They also express the BRCA-1, erbB2, and EGF receptor genes and possess the H-ras, K-ras, and p53 genes. Preliminary data showed that one of the new cancer cell lines was highly sensitive to the cytotoxic action of taxol. It is envisioned that the new breast cell lines will be useful as targets for identification of therapeutic agents against breast cancer and as models for carcinogenesis studies.
新的人乳腺细胞系是从转移性乳腺癌组织和正常乳腺组织中培养出来的。原代培养是从外植组织的细胞生长物或两种无血清培养基中机械分离的细胞开始的。来自癌症组织和正常组织的细胞培养物用pRSV-T质粒永生化，以产生呈现上皮形态的永久性乳腺细胞系。本研究中产生的细胞系在形态、生长速率、核型、特定基因的存在以及上皮和乳腺标志物的表达方面进行了表征。这些细胞系表达上皮细胞标志物细胞角蛋白8和18，并保留了产生人乳脂肪球蛋白的能力。它们还表达BRCA-1、erbB2和EGF受体基因，并拥有H-ras、K-ras和p53基因。初步数据表明，其中一种新的癌细胞系对紫杉醇的细胞毒性作用高度敏感。预计新的乳腺细胞系将作为鉴定抗乳腺癌治疗药物的靶点以及致癌研究的模型。
