Iype P T, Iype L E, Verma M, Kaighn M E
Biological Research Faculty and Facility, Inc., 10075-20 Tyler Place, Ijamsville, Maryland 21754, USA.
Int J Oncol. 1998 Feb;12(2):257-63. doi: 10.3892/ijo.12.2.257.
New human prostate cell lines were developed from prostatic carcinoma (BRF-41T) and BPH (BRF-55T). Primary cultures were initiated from cellular outgrowths of explanted tissues. A serum-free medium, BRFF-HPC1, was developed for growing human prostatic cancer cells. Cell strains were immortalized with pRSV-T plasmid to generate permanent cell lines that exhibited an epithelial morphology. Both cell lines expressed the epithelial cell markers, cytokeratins 8 and 18 as well as the prostatic marker, PSA, and the androgen receptor gene. They possess the H-ras, K-ras, and p53 genes. We hope that these new human prostatic cell lines will be useful as in vitro models for cancer research.
