Dufour A, Corsini E, Gelati M, Ciusani E, Zaffaroni M, Giombini S, Massa G, Salmaggi A
Istituto Nazionale Neurologico C. Besta, Milano, Italy.
J Neurol Sci. 1998 May 7;157(2):117-21. doi: 10.1016/s0022-510x(98)00059-8.
The in vitro effects of dexamethasone (Dx) and low and high-dose 6-methylprednisolone (MP) on the expression of adhesion molecules ICAM-1,VCAM-1 and class II antigen HLA-DR on human brain endothelial cells (HBECs) was studied. HBECs were obtained from the surgical specimen of a multiple sclerosis patient undergoing brain surgery for vascular aneurysm. HBECs obtained from apparently normal brain capillaries of surgical specimens of two patients undergoing brain surgery for a meningioma and a low-grade glioma, respectively, were used as controls. The effect of steroids was studied both in the basal condition and after stimulation with proinflammatory cytokines (gamma-IFN and TNF-alpha). In order to detect possible endothelium local tissue specific differences, the experiment was repeated using human umbilical vein endothelial cells (HUVECs). Only high-dose MP was able to down-regulate TNF-alpha-induced VCAM-1 expression on endothelial cells.
研究了地塞米松（Dx）以及低剂量和高剂量6-甲基泼尼松龙（MP）对人脑血管内皮细胞（HBECs）上黏附分子ICAM-1、VCAM-1和II类抗原HLA-DR表达的体外作用。HBECs取自一名因血管性动脉瘤接受脑部手术的多发性硬化症患者的手术标本。分别取自两名因脑膜瘤和低度胶质瘤接受脑部手术患者手术标本中明显正常脑毛细血管的HBECs用作对照。在基础条件下以及用促炎细胞因子（γ-干扰素和肿瘤坏死因子-α）刺激后研究了类固醇的作用。为了检测可能存在的内皮局部组织特异性差异，使用人脐静脉内皮细胞（HUVECs）重复了该实验。只有高剂量MP能够下调肿瘤坏死因子-α诱导的内皮细胞上VCAM-1的表达。
