Pei R, Wang G, Tarsitani C, Rojo S, Chen T, Takemura S, Liu A, Lee J
Department of Research, One Lambda, Inc., Canoga Park, CA 91303, USA.
Hum Immunol. 1998 May;59(5):313-22. doi: 10.1016/s0198-8859(98)00020-2.
A flow cytometric method of simultaneously screening both HLA Class I and Class II panel reactive antibodies (PRA) was developed using a pool of 30 different Class I and 30 different Class II microbeads coated with purified HLA antigens. The antibodies in the serum that react specifically to the coated HLA antigens are detected by using a FITC-conjugated antibody against human IgG. Percent PRA can be determined by the percentage of microbeads that react positively to the serum. There is no cross-reactivity between the Class I and Class II microbeads. A mixture of Class I and Class II microbeads can be distinguished by their different fluorescent properties on the flow cytometry analysis. Thus, both Class I and Class II PRA can be detected from a single tube reaction.
