Peek R, Verbraak F, Bruinenberg M, Van der Lelij A, Van den Horn G, Kijlstra A
Netherlands Ophthalmic Research Institute, Amsterdam.
Invest Ophthalmol Vis Sci. 1998 Jun;39(7):1183-7.
To determine the frequency of cytomegalovirus glycoprotein B (gB) genotypes in clinical samples of ocular fluids of patients with acquired immune deficiency syndrome (AIDS) who have cytomegalovirus retinitis and to compare these with the cytomegalovirus gB genotype in paired peripheral blood leukocytes.
Glycoprotein B genotypes of cytomegalovirus genomic DNA were determined in 29 ocular and 9 paired blood samples of 27 patients, by polymerase chain reaction amplification followed by restriction fragment length polymorphism analysis.
In the 29 ocular samples, 30 gB genotypes were determined: Glycoprotein B1 was found in 8 samples (27%), gB2 in 9 samples (30%), gB3 in 6 samples (20%), and gB4 in 3 samples (10%). In one sample, a mixed genotype was observed. In addition to these previously characterized gB genotypes, a new gB variant was observed in the ocular fluid of four patients. Partial sequence analysis revealed that this new gB genotype is closely related to gB3, and it was therefore named gB3'. In the blood samples, only gB1, gB2, and gB3 genotypes were observed. In the nine paired samples of ocular fluid and blood, four showed a difference in gB genotype between these compartments.
The distribution of cytomegalovirus glycoprotein B genotypes gB1-gB4 in ocular fluids of patients with AIDS who have cytomegalovirus retinitis was determined in this study. The predominance of gB2, as described by others, was not confirmed. The glycoprotein B genotype in the eye can be different from the genotype found in the blood of the same patient. A new gB variant, gB3', was found in the ocular samples of 4 of 27 patients, but not in the blood samples tested.
确定患有巨细胞病毒性视网膜炎的获得性免疫缺陷综合征（AIDS）患者眼液临床样本中巨细胞病毒糖蛋白B（gB）基因型的频率，并将其与配对的外周血白细胞中的巨细胞病毒gB基因型进行比较。
通过聚合酶链反应扩增，随后进行限制性片段长度多态性分析，在27例患者的29份眼液样本和9份配对血液样本中确定巨细胞病毒基因组DNA的糖蛋白B基因型。
在29份眼液样本中，确定了30种gB基因型：8份样本（27%）中发现糖蛋白B1，9份样本（30%）中发现gB2，6份样本（20%）中发现gB3，3份样本（10%）中发现gB4。在一份样本中观察到混合基因型。除了这些先前已鉴定的gB基因型外，在4例患者的眼液中观察到一种新的gB变异体。部分序列分析显示，这种新的gB基因型与gB3密切相关，因此命名为gB3'。在血液样本中，仅观察到gB1、gB2和gB3基因型。在9份配对的眼液和血液样本中，4份在这些样本区间的gB基因型存在差异。
本研究确定了患有巨细胞病毒性视网膜炎的AIDS患者眼液中巨细胞病毒糖蛋白B基因型gB1 - gB4的分布。未证实其他人所描述的gB2的优势地位。眼睛中的糖蛋白B基因型可能与同一患者血液中发现的基因型不同。在27例患者中的4例眼液样本中发现了一种新的gB变异体gB3'，但在检测的血液样本中未发现。
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