大鼠下丘脑中的焦谷氨酸-N3-亚氨基甲基-组氨酰-脯氨酰胺（甲基促甲状腺激素释放激素）
Pyroglutamyl-N3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone) in the rat hypothalamus.
作者信息
Pekary A E, Morley J E, Hershman J M
出版信息
J Endocrinol. 1978 Jun;77(3):405-8. doi: 10.1677/joe.0.0770405.
Pyroglutamyl-N3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone, methyl-TRH) is a potent synthetic analogue of TRH. N3im-Methyl-histidine is present in mammalian brain and it has been suggested that methyl-TRH is a physiological releasing hormone normally present in the hypothalamus. A non-gradient cation-exchange chromatography system that uses SP-Sephadex C-25 and completely resolves methyl-TRH and TRH has been developed. Because methyl-TRH cross-reacts in the immunoassay for TRH, this assay was used to measure TRH and methyl-TRH in the chromatographic fractions. By this means it has been demonstrated that the amount of methyl-TRH present in the rat is less than 0.025 ng/hypothalamus.
焦谷氨酸-N3im-甲基-组氨酰-脯氨酰胺（甲基促甲状腺激素释放激素，甲基-TRH）是TRH的一种强效合成类似物。N3im-甲基组氨酸存在于哺乳动物大脑中，有人提出甲基-TRH是下丘脑正常存在的一种生理性释放激素。已开发出一种使用SP-葡聚糖凝胶C-25的非梯度阳离子交换色谱系统，该系统能完全分离甲基-TRH和TRH。由于甲基-TRH在TRH免疫测定中有交叉反应，因此该测定法用于测量色谱馏分中的TRH和甲基-TRH。通过这种方法已证明，大鼠下丘脑内甲基-TRH的含量低于0.025 ng/下丘脑。
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