Moreira-Ferreira A M, Kalapothakis E, Diniz C R, Chávez-Olórtegui C
Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Toxicon. 1998 Feb;36(2):333-9. doi: 10.1016/s0041-0101(97)00103-7.
The possibility of inducing a humoral immune response able to produce neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model. A non-toxic protein (TsNTxP) was purified from the venom of the Brazilian scorpion Tityus serrulatus by combining gel filtration, ion exchange and reverse phase HPLC chromatographic steps. After four injections of TsNTxP the mice developed an IgG response. The anti-TsNTxP antibodies had a comparable high cross-reactivity for the crude venom, toxic fraction (toxic fraction of venom that represents most of the toxicity of the crude venom -- TsTFG50) and TsIV, a representative alpha-type toxin of T. serrulatus, and moderate binding capacity for TsVII, a representative beta-type toxin. In vitro neutralization assays indicated that preincubation of a lethal dose of the toxic fraction with immune serum strongly reduced its toxicity. In vivo protection assays showed that mice immunized with TsNTxP resisted a challenge of 10 LD50 (s.c.) of the toxic fraction of T. serrulatus venom.
在小鼠模型中评估了诱导体液免疫反应以产生针对蝎毒素致死效应的中和抗体的可能性。通过结合凝胶过滤、离子交换和反相高效液相色谱步骤，从巴西蝎子锯齿脂鲤蝎的毒液中纯化出一种无毒蛋白质（TsNTxP）。在四次注射TsNTxP后，小鼠产生了IgG反应。抗TsNTxP抗体对粗毒液、有毒部分（代表粗毒液大部分毒性的毒液有毒部分——TsTFG50）和TsIV（锯齿脂鲤蝎的代表性α型毒素）具有相当高的交叉反应性，对TsVII（代表性β型毒素）具有中等结合能力。体外中和试验表明，用免疫血清对致死剂量的有毒部分进行预孵育可大大降低其毒性。体内保护试验表明，用TsNTxP免疫的小鼠能够抵抗10倍半数致死剂量（皮下注射）的锯齿脂鲤蝎毒液有毒部分的攻击。
