Lahdenpohja N, Hurme M
Department of Microbiology and Immunology, University of Tampere Medical School, Finland.
J Leukoc Biol. 1998 Jun;63(6):775-80. doi: 10.1002/jlb.63.6.775.
We have analyzed the effect of complete T cell activation (anti-CD3 plus anti-CD28) on the activation of NF-kappaB in CD45RA+ (naive) and CD45RO+ (memory/effector) T cells. Long exposure (24 h) induced stronger NF-kappaB DNA binding in CD45RA+ cells than in CD45RO+ cells. Analysis of the nuclear c-Rel protein indicated that after anti-CD3+anti-CD28 stimulation the level of c-Rel was higher in CD45RA+ cells. Analysis of the cytoplasmic inhibitor IkappaBalpha indicated that anti-CD3+anti-CD28 stimulation induced a long-lasting degradation in CD45RA+ cells but in CD45RO+ cells the degradation process was more rapid. Because the CD28 costimulus is known to induce the production of reactive oxygen intermediates (ROIs), the intracellular ROI levels in CD45RA+ and CD45RO+ cells were compared by flow cytometry. ROIs were produced in both cell types, but more strongly in CD45RA+ cells. The data presented in this study further emphasize the differences between CD45RA+ and CD45RO+ T lymphocytes in ROI-dependent signaling pathways.
我们分析了完全T细胞激活（抗CD3加抗CD28）对CD45RA +（初始）和CD45RO +（记忆/效应）T细胞中NF-κB激活的影响。长时间暴露（24小时）诱导CD45RA +细胞中比CD45RO +细胞更强的NF-κB DNA结合。对核c-Rel蛋白的分析表明，抗CD3 +抗CD28刺激后，CD45RA +细胞中c-Rel水平更高。对细胞质抑制剂IκBα的分析表明，抗CD3 +抗CD28刺激在CD45RA +细胞中诱导了持久的降解，但在CD45RO +细胞中降解过程更快。由于已知CD28共刺激可诱导活性氧中间体（ROI）的产生，因此通过流式细胞术比较了CD45RA +和CD45RO +细胞中的细胞内ROI水平。两种细胞类型均产生ROI，但在CD45RA +细胞中产生更强。本研究中呈现的数据进一步强调了CD45RA +和CD45RO + T淋巴细胞在ROI依赖性信号通路中的差异。
