Cannio R, Contursi P, Rossi M, Bartolucci S
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
J Bacteriol. 1998 Jun;180(12):3237-40. doi: 10.1128/JB.180.12.3237-3240.1998.
A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C.
通过将病毒粒子SSV1的自主复制序列和hph(潮霉素磷酸转移酶)基因的合适突变体作为转化标记插入大肠杆菌质粒中,构建了一种能够在嗜热栖热菌和大肠杆菌中转化并稳定维持的质粒。该载体没有发生重排和/或染色体整合,并且通过将细胞暴露于丝裂霉素C,其在嗜热栖热菌中的拷贝数增加。