C蛋白对噬菌体Mu mom转录的激活并不需要与大肠杆菌RNA聚合酶α或σ70亚基的羧基末端区域进行特异性相互作用。
Activation of bacteriophage Mu mom transcription by C protein does not require specific interaction with the carboxyl-terminal region of the alpha or sigma 70 subunit of Escherichia coli RNA polymerase.
作者信息
Sun W, Hattman S, Fujita N, Ishihama A
机构信息
Department of Biology, University of Rochester, New York 14627, USA.
出版信息
J Bacteriol. 1998 Jun;180(12):3257-9. doi: 10.1128/JB.180.12.3257-3259.1998.
Abstract
Late in its growth cycle, transcription of the phage Mu mom Promoter (Pmom) is activated by the phage gene product, C, a site-specific DNA binding protein. In vitro transcription analyses showed that this activation does not require specific contacts between C and the carboxyl-terminal region of the alpha or sigma 70 subunit of Escherichia coli RNA polymerase. Unexpectedly, these results are in contrast to those known for another Mu-encoded transcriptional activator, Mor, which has a high degree of sequence identity with C and appears to interact with the carboxyl termini of both alpha and sigma 70.
摘要
在其生长周期后期，噬菌体Mu的妈妈启动子（Pmom）的转录由噬菌体基因产物C激活，C是一种位点特异性DNA结合蛋白。体外转录分析表明，这种激活不需要C与大肠杆菌RNA聚合酶α或σ70亚基的羧基末端区域之间的特异性接触。出乎意料的是，这些结果与另一种Mu编码的转录激活因子Mor的已知结果相反，Mor与C具有高度的序列同一性，并且似乎与α和σ70的羧基末端相互作用。
相似文献
1
Activation of bacteriophage Mu mom transcription by C protein does not require specific interaction with the carboxyl-terminal region of the alpha or sigma 70 subunit of Escherichia coli RNA polymerase.C蛋白对噬菌体Mu mom转录的激活并不需要与大肠杆菌RNA聚合酶α或σ70亚基的羧基末端区域进行特异性相互作用。
J Bacteriol. 1998 Jun;180(12):3257-9. doi: 10.1128/JB.180.12.3257-3259.1998.
2
Bidirectional transcription in the mom promoter region of bacteriophage Mu.噬菌体Mu的mom启动子区域中的双向转录
J Mol Biol. 1998 Dec 11;284(4):885-92. doi: 10.1006/jmbi.1998.2228.
3
Transcriptional activator C protein-mediated unwinding of DNA as a possible mechanism for mom gene activation.转录激活因子C蛋白介导的DNA解旋作为mom基因激活的一种可能机制。
J Mol Biol. 1998 Dec 11;284(4):893-902. doi: 10.1006/jmbi.1998.2213.
4
Mutations in β' subunit of Escherichia coli RNA polymerase perturb the activator polymerase functional interaction required for promoter clearance.大肠杆菌 RNA 聚合酶β'亚基突变扰乱了激活剂聚合酶功能相互作用，该相互作用对于启动子清除是必需的。
Mol Microbiol. 2011 Jun;80(5):1169-85. doi: 10.1111/j.1365-2958.2011.07636.x. Epub 2011 Apr 5.
5
Interaction of the bacteriophage Mu transcriptional activator protein, C, with its target site in the mom promoter.噬菌体Mu转录激活蛋白C与其在mom启动子中的靶位点之间的相互作用。
J Mol Biol. 1997 Nov 7;273(4):765-74. doi: 10.1006/jmbi.1997.1349.
6
Escherichia coli OxyR protein represses the unmethylated bacteriophage Mu mom operon without blocking binding of the transcriptional activator C.大肠杆菌OxyR蛋白抑制未甲基化的噬菌体Mu mom操纵子，而不阻止转录激活因子C的结合。
Nucleic Acids Res. 1996 Oct 15;24(20):4042-9. doi: 10.1093/nar/24.20.4042.
7
DNA unwinding mechanism for the transcriptional activation of momP1 promoter by the transactivator protein C of bacteriophage Mu.
J Biol Chem. 2001 Dec 14;276(50):46941-5. doi: 10.1074/jbc.M107476200. Epub 2001 Oct 11.
8
Different Modes of Transactivation of Bacteriophage Mu Late Promoters by Transcription Factor C.转录因子C对噬菌体Mu晚期启动子的不同反式激活模式
PLoS One. 2015 Jun 9;10(6):e0129504. doi: 10.1371/journal.pone.0129504. eCollection 2015.
9
Bacteriophage Mu Mor protein requires sigma 70 to activate the Mu middle promoter.噬菌体Mu的Mor蛋白需要σ70来激活Mu中间启动子。
J Bacteriol. 1993 Sep;175(17):5314-23. doi: 10.1128/jb.175.17.5314-5323.1993.
10
Activation of the bacteriophage Mu lys promoter by Mu C protein requires the sigma 70 subunit of Escherichia coli RNA polymerase.噬菌体Mu裂解启动子的激活需要大肠杆菌RNA聚合酶的σ70亚基参与，这一过程由Mu C蛋白介导。
J Bacteriol. 1990 Mar;172(3):1424-9. doi: 10.1128/jb.172.3.1424-1429.1990.
引用本文的文献
1
Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding.编码噬菌体Mu晚期基因激活剂C的基因的区域诱变鉴定出两个对DNA结合很重要的独立区域。
Nucleic Acids Res. 2008 Nov;36(20):6396-405. doi: 10.1093/nar/gkn639. Epub 2008 Oct 5.
本文引用的文献
1
Interaction of the bacteriophage Mu transcriptional activator protein, C, with its target site in the mom promoter.噬菌体Mu转录激活蛋白C与其在mom启动子中的靶位点之间的相互作用。
J Mol Biol. 1997 Nov 7;273(4):765-74. doi: 10.1006/jmbi.1997.1349.
2
Region 2.5 of the Escherichia coli RNA polymerase sigma70 subunit is responsible for the recognition of the 'extended-10' motif at promoters.大肠杆菌RNA聚合酶σ70亚基的2.5区域负责识别启动子处的“扩展-10”基序。
EMBO J. 1997 Jul 1;16(13):4034-40. doi: 10.1093/emboj/16.13.4034.
3
Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both alpha and sigma70 subunits of Escherichia coli RNA polymerase.噬菌体Mu Mor蛋白的转录激活需要大肠杆菌RNA聚合酶α亚基和σ70亚基的C末端区域。
J Biol Chem. 1996 Dec 13;271(50):32343-8. doi: 10.1074/jbc.271.50.32343.
4
Escherichia coli OxyR protein represses the unmethylated bacteriophage Mu mom operon without blocking binding of the transcriptional activator C.大肠杆菌OxyR蛋白抑制未甲基化的噬菌体Mu mom操纵子，而不阻止转录激活因子C的结合。
Nucleic Acids Res. 1996 Oct 15;24(20):4042-9. doi: 10.1093/nar/24.20.4042.
5
Reconstitution of RNA polymerase.RNA聚合酶的重组
Methods Enzymol. 1996;273:121-30. doi: 10.1016/s0076-6879(96)73011-2.
6
Sequence-specific DNA binding of the phage Mu C protein: footprinting analysis reveals altered DNA conformation upon protein binding.噬菌体Mu C蛋白的序列特异性DNA结合：足迹分析揭示了蛋白质结合后DNA构象的改变。
J Mol Biol. 1996 Jul 5;260(1):22-33. doi: 10.1006/jmbi.1996.0379.
7
Regulatory factors acting at the bacteriophage Mu middle promoter.作用于噬菌体Mu中间启动子的调控因子。
J Bacteriol. 1996 Mar;178(6):1585-92. doi: 10.1128/jb.178.6.1585-1592.1996.
8
Transcription activation by the bacteriophage Mu Mor protein: analysis of promoter mutations in Pm identifies a new region required for promoter function.噬菌体Mu Mor蛋白介导的转录激活：Pm中启动子突变分析确定了启动子功能所需的一个新区域。
Nucleic Acids Res. 1996 Feb 1;24(3):450-7. doi: 10.1093/nar/24.3.450.
9
The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an "extended minus 10" promoter.大肠杆菌σ70的-35识别区域对于在“延伸的-10”启动子处起始转录并非必需。
J Mol Biol. 1993 Jul 20;232(2):406-18. doi: 10.1006/jmbi.1993.1400.
10
A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase.细菌启动子中的第三种识别元件：RNA聚合酶α亚基与DNA的结合。
Science. 1993 Nov 26;262(5138):1407-13. doi: 10.1126/science.8248780.
Zotero 插件