Wachtel S S, Sammons D, Twitty G, Utermohlen J, Tolley E, Phillips O, Shulman L P
Department of Obstetrics & Gynecology, University of Tennessee, Memphis 38103, USA.
Prenat Diagn. 1998 May;18(5):455-63.
We set out to ascertain the numbers of fetal cells that enter the maternal blood stream during pregnancy. Samples of 15-16 ml of whole blood were collected from 225 women--mostly 10-18 weeks pregnant--and then processed by charge flow separation, a novel method based on free flow electrophoresis in a buffer counterflow gradient. After their recovery in four different separation instruments, nucleated red blood cells (NRBC) were enumerated histologically. In some cases fetal NRBC were identified and enumerated by fluorescence in situ hybridization with probes for the X and Y chromosomes and fetal haemoglobin mRNA. Recoveries were consistent among the four separation instruments: the median numbers of NRBC obtained were 4190, 1590, 2805 and 3860. Our data show that approximately 30 per cent of those cells were fetal. Thus, recent reports on the separation of fetal NRBC by other methods, give underestimates of their frequency in maternal blood.
我们着手确定孕期进入母体血流的胎儿细胞数量。从225名女性（大多怀孕10 - 18周）采集15 - 16毫升全血样本，然后通过电荷流分离法进行处理，这是一种基于缓冲逆流梯度中自由流动电泳的新方法。在四种不同的分离仪器中回收后，通过组织学方法对有核红细胞（NRBC）进行计数。在某些情况下，通过用X和Y染色体及胎儿血红蛋白mRNA探针进行荧光原位杂交来鉴定和计数胎儿NRBC。四种分离仪器的回收率一致：获得的NRBC中位数分别为4190、1590、2805和3860。我们的数据表明，这些细胞中约30%为胎儿细胞。因此，近期关于用其他方法分离胎儿NRBC的报告低估了它们在母体血液中的频率。
