阿尔茨海默病患者皮肤成纤维细胞中早老素-1 mRNA的表达

The expression of presenilin-1 mRNA in skin fibroblasts from Alzheimer's disease.

作者信息

Ikeda K, Urakami K, Isoe K, Ohno K, Nakashima K

机构信息

Division of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, Yonago, Japan.

出版信息

Dement Geriatr Cogn Disord. 1998 May-Jun;9(3):145-8. doi: 10.1159/000017039.

DOI:10.1159/000017039

PMID:9622002

Abstract

The presenilin-1 (PS-1) gene was recently identified as one of the causative genes in the early onset of familial Alzheimer's disease (AD). Analysis of the PS-1 gene is thought to be useful in clarifying the pathogenesis of AD. However, there have been few reports about the expression of the PS-1 gene in AD. In this study, we analyzed the expression of PS-1 mRNA in cultured skin fibroblasts taken from living patients with AD by Northern blot analysis. The subjects consisted of 18 cases with AD and 10 cases of neurological patients without dementia (CTL). We found that the PS-1 mRNA levels in AD were significantly higher than those in CTL (p < 0.01). Moreover, we found that the PS-1 mRNA level increases in the early stages of AD and tends to decrease in the advanced stages. These findings suggest that high levels of PS-1 mRNA may play an important role in developing AD.

摘要

早老素-1（PS-1）基因最近被确定为家族性早发性阿尔茨海默病（AD）的致病基因之一。PS-1基因分析被认为有助于阐明AD的发病机制。然而，关于PS-1基因在AD中的表达报道较少。在本研究中，我们通过Northern印迹分析，分析了从AD活体患者获取的培养皮肤成纤维细胞中PS-1 mRNA的表达。研究对象包括18例AD患者和10例无痴呆的神经科患者（CTL）。我们发现AD患者的PS-1 mRNA水平显著高于CTL组（p < 0.01）。此外，我们发现PS-1 mRNA水平在AD早期升高，在晚期趋于下降。这些发现表明，高水平的PS-1 mRNA可能在AD的发生发展中起重要作用。