Morishita R, Higaki J, Tomita N, Ogihara T
Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan.
Circ Res. 1998 Jun 1;82(10):1023-8. doi: 10.1161/01.res.82.10.1023.
Recent progress in molecular biology has provided new techniques for inhibiting target gene expression. In particular, the application of DNA technology, such as antisense strategy to regulate the transcription of disease-related genes in vivo, has important therapeutic potential. Recently, transfection of cis-element double-stranded oligodeoxynucleotides (ODNs), referred to as "decoy" ODNs, has been reported to be a powerful tool in a new class of anti-gene strategies for gene therapy and in the study of transcriptional regulation. Transfection of double-stranded ODNs corresponding to the cis sequence will result in the attenuation of authentic cis-trans interaction, leading to the removal of trans factors from the endogenous cis elements with subsequent modulation of gene expression. This "decoy" strategy is not only a novel strategy for gene therapy as an anti-gene strategy but also a powerful tool for the study of endogenous gene regulation in vivo as well as in vitro. In this article, we reviewed (1) the mechanisms and (2) the potential applications of decoy strategy.
分子生物学的最新进展为抑制靶基因表达提供了新技术。特别是DNA技术的应用，如反义策略在体内调节疾病相关基因的转录，具有重要的治疗潜力。最近，顺式元件双链寡脱氧核苷酸（ODN）（称为“诱饵”ODN）的转染已被报道是一类新型的基因治疗抗基因策略以及转录调控研究中的有力工具。转染与顺式序列对应的双链ODN将导致真实的顺式-反式相互作用减弱，从而使反式因子从内源性顺式元件上脱离，进而调节基因表达。这种“诱饵”策略不仅是一种作为抗基因策略的新型基因治疗方法，也是体内外研究内源性基因调控的有力工具。在本文中，我们综述了（1）诱饵策略的机制和（2）其潜在应用。
