Burrows W M, Stoughton R B
Arch Dermatol. 1976 Feb;112(2):175-8.
Ten healthy human volunteers were exposed to a primary sensitizing dose of 1 mg dinitrochlorobenzene (DNCB) by an open topical technique within an area that had been pretreated with a potent topical glucocorticosteroid compound. Quantitative elicitation testing was performed on the opposite side by an open patch test technique two weeks after the sensitizing application. One (10%) of the ten subjects became sensitized. A matched control group of ten subjects was similarly sensitized without steroid treatment. Eight (80%) of the ten became sensitized. One month later, five of the eight test subjects in whom sensitization had been prevented were retested in an identical fashion without steroid pretreatment, to determine if any degree of tolerance had been induced. All five subjects became sensitized. Topical glucocorticosteroids inhibited the development of sensitization to topically applied DNCB. Tolerance was not induced by this single process.
十名健康的人类志愿者通过开放局部应用技术，在已用强效局部糖皮质激素化合物预处理过的区域内，接触1毫克二硝基氯苯（DNCB）的初次致敏剂量。致敏应用两周后，通过开放斑贴试验技术在对侧进行定量激发试验。十名受试者中有一名（10%）致敏。一组由十名受试者组成的匹配对照组在未接受类固醇治疗的情况下进行了类似的致敏处理。十名中有八名（80%）致敏。一个月后，对八名原本致敏被预防的测试受试者中的五名，在未进行类固醇预处理的情况下以相同方式重新测试，以确定是否诱导出了任何程度的耐受性。所有五名受试者均致敏。局部糖皮质激素抑制了对局部应用DNCB致敏的发生。这一单一过程未诱导出耐受性。
