Ozaki K, Nagata M, Suzuki M, Fujiwara T, Miyoshi Y, Ishikawa O, Ohigashi H, Imaoka S, Takahashi E, Nakamura Y
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd. Tokushima, Japan.
Genes Chromosomes Cancer. 1998 Jul;22(3):179-85.
By means of the differential display method, we isolated a novel human gene that is expressed specifically in pancreas. The cDNA, designated "pancpin," contained an open reading frame of 1,215 nucleotides encoding a 405 amino acid protein, showing a high degree of similarity to serine protease inhibitors belonging to the serpin superfamily. To investigate its possible role in pancreatic carcinogenesis, we looked for genetic alterations of this gene in pancreatic cancer cell lines and primary pancreatic cancer tissues. Expression of pancpin was barely detectable in any of the four pancreatic cancer cell lines examined, and very weak also in 10 of 13 pancreatic cancer tissues. A somatic missense mutation at codon 221 was found in two of 16 primary pancreatic cancers. These findings indicate that down-regulation of pancpin expression may play a significant role in development or progression of pancreatic cancer.
通过差异显示法，我们分离出一个在胰腺中特异性表达的新型人类基因。该cDNA被命名为“pancpin”，包含一个1215个核苷酸的开放阅读框，编码一个405个氨基酸的蛋白质，与丝氨酸蛋白酶抑制剂超家族中的丝氨酸蛋白酶抑制剂具有高度相似性。为了研究其在胰腺癌发生中的可能作用，我们在胰腺癌细胞系和原发性胰腺癌组织中寻找该基因的遗传改变。在所检测的四个胰腺癌细胞系中，几乎检测不到pancpin的表达，在13个胰腺癌组织中的10个中表达也非常微弱。在16例原发性胰腺癌中的2例中发现了第221位密码子的体细胞错义突变。这些发现表明，pancpin表达的下调可能在胰腺癌的发生或发展中起重要作用。
