Emori M, Tomioka H, Sato K, Saito H
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
Int J Antimicrob Agents. 1998 Apr;10(1):59-65. doi: 10.1016/s0924-8579(98)00010-7.
In this study, profiles of infection due to Mycobacterium avium complex (MAC) in CB-17 SCID mice deficient in T and B cell functions were examined, when mice were given or not given a new benzoxazinorifamycin, KRM-1648 (KRM), during the course of infection. When mice were infected intravenously with MAC, the bacterial loads in their visceral organs were larger than those of their co-isogenic CB-17 counterparts. The incidence and the degree of gross lung lesions were less in SCID mice compared to CB-17 mice. Athymic BALB/c nude mice showed similar profiles of the infection. Beige mice showed more severe gross lesions and larger bacterial loads in the lungs than did SCID and athymic BALB/c nude mice. When MAC was infected subcutaneously into the hind footpads of mice, disseminated growth of organisms in the footpads, blood, and visceral organs was seen in SCID mice, but not in CB-17 or BALB/c mice. KRM exhibited the same level of therapeutic effect on SCID mice infected with MAC via the intravenous route in terms of inhibiting bacterial growth in the lungs and kidneys, as in cases of CB-17 and BALB/c mice with normal T-cell functions. In beige mice, the degree of growth inhibition of MAC due to KRM treatment was significantly greater than that achieved in SCID mice.
在本研究中，检测了T和B细胞功能缺陷的CB-17 SCID小鼠感染鸟分枝杆菌复合群（MAC）的情况，在感染过程中给小鼠或不给小鼠新的苯并恶嗪诺里霉素KRM-1648（KRM）。当小鼠静脉注射感染MAC时，其内脏器官中的细菌载量高于同基因的CB-17对照小鼠。与CB-17小鼠相比，SCID小鼠肺部大体病变的发生率和程度较低。无胸腺BALB/c裸鼠表现出相似的感染情况。米色小鼠肺部的大体病变比SCID和无胸腺BALB/c裸鼠更严重，细菌载量更大。当将MAC皮下注射到小鼠后足垫时，在SCID小鼠中可见细菌在足垫、血液和内脏器官中播散生长，但在CB-17或BALB/c小鼠中未见到。在抑制肺部和肾脏细菌生长方面，KRM对静脉注射感染MAC的SCID小鼠的治疗效果与T细胞功能正常的CB-17和BALB/c小鼠相同。在米色小鼠中，KRM治疗对MAC的生长抑制程度明显大于SCID小鼠。
