Hinton P S, Peterson C A, McCarthy D O, Ney D M
Department of Nutritional Sciences and School of Nursing, University of Wisconsin, Madison 53706, USA.
Am J Clin Nutr. 1998 Jun;67(6):1265-72. doi: 10.1093/ajcn/67.6.1265.
We tested whether infusion of medium-chain triacylglycerols (MCTs) during total parenteral nutrition (TPN) enhanced macrophage response and reduced intestinal atrophy compared with long-chain triacylglycerols (LCTs). Male Sprague-Dawley rats (230-240 g) were maintained with TPN providing 16% or 48% of nonprotein energy from MCTs plus LCTs or LCTs alone or 100% of nonprotein energy from dextrose for 6 or 12 d. Body weight gain was not significantly different among groups. Serum concentrations of beta-hydroxybutyrate were greater with MCTs plus LCTs than with LCTs alone after 6 d (P < 0.05, main effect). Triacylglycerol concentrations in liver were greater with LCTs than with MCTs plus LCTs after 6 or 12 d (P < 0.05, main effect). MCTs plus LCTs increased by 50% the percentage (P < 0.0005) and number of splenic macrophages compared with LCTs alone in conjunction with decreased triacylglycerol concentrations in spleen after 6 d (P < 0.05, main effect). In vitro tumor necrosis factor alpha secretion by splenic or circulating macrophages in response to lipopolysaccharide was increased by MCTs plus LCTs compared with LCTs alone, twofold after 6 and sevenfold after 12 d (P < 0.05, main effect). Jejunal mucosal mass was 30% greater with MCTs plus LCTs than with LCTs alone after 6 or 12 d (P < 0.01); villus height was also significantly greater after 6 d (main effect). The incidence of bacterial translocation to the mesenteric lymph nodes was not significantly different among groups. Compared with LCTs, MCTs enhanced macrophage response and decreased intestinal atrophy.
我们测试了在全肠外营养（TPN）期间输注中链甘油三酯（MCTs）与长链甘油三酯（LCTs）相比，是否能增强巨噬细胞反应并减少肠道萎缩。将雄性Sprague-Dawley大鼠（230 - 240克）用TPN维持，其中TPN提供来自MCTs加LCTs或单独LCTs的16%或48%的非蛋白能量，或来自葡萄糖的100%非蛋白能量，持续6或12天。各组间体重增加无显著差异。6天后，MCTs加LCTs组的血清β-羟基丁酸浓度高于单独LCTs组（P < 0.05，主效应）。6或12天后，LCTs组肝脏中的甘油三酯浓度高于MCTs加LCTs组（P < 0.05，主效应）。与单独LCTs相比，MCTs加LCTs使脾脏巨噬细胞的百分比（P < 0.0005）和数量增加了50%，同时6天后脾脏中的甘油三酯浓度降低（P < 0.05，主效应）。与单独LCTs相比，MCTs加LCTs使脾脏或循环巨噬细胞对脂多糖刺激的体外肿瘤坏死因子α分泌增加，6天后增加两倍，12天后增加七倍（P < 0.05，主效应）。6或12天后，MCTs加LCTs组的空肠黏膜质量比单独LCTs组大30%（P < 0.01）；6天后绒毛高度也显著更高（主效应）。各组间细菌易位至肠系膜淋巴结的发生率无显著差异。与LCTs相比，MCTs增强了巨噬细胞反应并减少了肠道萎缩。
