Stomper P C, DeBloom J R, Budnick R M, Stewart C C
Division of Diagnostic Imaging, Roswell Park Cancer Institute, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14263, USA.
Clin Cancer Res. 1998 Jun;4(6):1543-7.
There is little information regarding flow cytometric DNA analyses of benign breast lesions. This prospective study consists of mammographic and pathological correlation of DNA flow cytometric analyses of specimen mammography-guided fine-needle aspirates (FNAs) of 189 consecutive benign breast lesions and 114 FNAs of adjacent normal tissue as a control. Clinical follow-up was also performed. Aneuploidy was detected in 14 of 189 (7%) benign lesion specimen mammography-guided FNAs and in only 1 of 114 (0.9%) FNAs of adjacent normal tissue (P = 0.01). Aneuploidy was detected in two (33%) benign intramammary lymph nodes compared with four (12%) benign lesions with atypia, one benign lesion (3%) with hyperplasia, four benign lesions (10%) with adenosis, and three (4%) other benign lesions (P = 0.01). There were no significant associations between DNA content and S-phase percentage and patient age, mammographic appearance, or extent. During a median follow-up of 40 months (range, 6-84 months), 2 of 13 (15%) patients with aneuploid benign lesions developed ipsilateral breast carcinoma compared with 5 of 175 (3%) patients with diploid benign lesions (odds ratio, 6.18; 95% confidence interval, 1.08-35.56). Our data suggest that aneuploidy, which is detected in a variety of benign breast lesions, may be associated with a higher risk of development of breast carcinoma. The combined techniques of specimen mammography-guided fine-needle aspiration and flow cytometry provide a practical translational research method for the study of benign breast disease.
关于良性乳腺病变的流式细胞术DNA分析的信息很少。这项前瞻性研究包括对189例连续的良性乳腺病变的标本进行乳腺X线摄影引导下细针穿刺抽吸（FNA）的DNA流式细胞术分析与病理的相关性，并以114例相邻正常组织的FNA作为对照。同时也进行了临床随访。在189例良性病变标本乳腺X线摄影引导下的FNA中，有14例（7%）检测到非整倍体，而在114例相邻正常组织的FNA中仅1例（0.9%）检测到非整倍体（P = 0.01）。与4例（12%）非典型良性病变、1例（3%）增生性良性病变、4例（10%）腺病性良性病变和3例（4%）其他良性病变相比，2例（33%）良性乳腺内淋巴结检测到非整倍体（P = 0.01）。DNA含量和S期百分比与患者年龄、乳腺X线表现或范围之间无显著关联。在中位随访40个月（范围6 - 84个月）期间，13例非整倍体良性病变患者中有2例（15%）发生同侧乳腺癌，而175例二倍体良性病变患者中有5例（3%）发生同侧乳腺癌（比值比，6.18；95%置信区间，1.08 - 35.56）。我们的数据表明，在多种良性乳腺病变中检测到的非整倍体可能与乳腺癌发生风险较高有关。标本乳腺X线摄影引导下细针抽吸和流式细胞术的联合技术为良性乳腺疾病的研究提供了一种实用的转化研究方法。
