Stomper P C, DeBloom J R, Winston J S, Stewart C C
Division of Diagnostic Imaging, Roswell Park Cancer Institute, School of Medicine and Biomedical Sciences, SUNY at Buffalo, NY 14263, USA.
J Exp Clin Cancer Res. 2000 Sep;19(3):309-15.
Flow cytometric studies of screening mammography detected ductal carcinoma in situ (DCIS) are limited by the lack of fresh cell samples. We have performed flow cytometric DNA analyses of specimen mammography-guided fine-needle aspirates of 50 consecutive DCIS lesions detected by screening mammography. The comedo histologic subtype had an aneuploidy rate of 39% (9 of 23); noncomedo subtypes had an aneuploidy rate of 19% (5 of 27), p=ns. Noncomedo subtypes were more likely to have low (less than 2.2%) S-phase percentages, 59% (16 of 27) as compared to comedo, 9% (2 of 23), p<0.05. High and intermediate nuclear grade DCIS lesions had an insignificantly greater rate of aneuploidy, 35% (9 of 26) and 33% (4 of 12) respectively, as compared to low nuclear grade lesions, 8% (1 of 12), p=ns. Low and intermediate nuclear grade DCIS lesions had low S-phase percentage rates of 67% and 50% respectively, as compared to the high nuclear grade lesions low S-phase percentage rate, 15%, p=ns. Aneuploidy and lesser rates of low S-phase percentages were significantly associated with necrosis and apoptosis. Our data suggest that flow cytometric DNA analysis of mammographic lesion-specific, fresh cell samples obtained by fine-needle aspiration under specimen mammographic guidance can assess mammography-detected DCIS lesions when gross fresh tissue procurement is not possible.
乳腺钼靶筛查发现的导管原位癌（DCIS）的流式细胞术研究因缺乏新鲜细胞样本而受到限制。我们对乳腺钼靶筛查发现的50例连续DCIS病变进行了乳腺钼靶引导下细针穿刺抽吸样本的流式细胞术DNA分析。粉刺型组织学亚型的非整倍体率为39%（23例中的9例）；非粉刺型亚型的非整倍体率为19%（27例中的5例），p值无统计学意义。非粉刺型亚型更有可能具有低（低于2.2%）的S期百分比，为59%（27例中的16例），而粉刺型为9%（23例中的2例），p<0.05。与低核级病变（8%，12例中的1例）相比，高核级和中核级DCIS病变的非整倍体率分别为35%（26例中的9例）和33%（12例中的4例），无显著差异，p值无统计学意义。与高核级病变的低S期百分比率（15%）相比，低核级和中核级DCIS病变的S期百分比率分别为67%和50%，p值无统计学意义。非整倍体和较低的低S期百分比率与坏死和凋亡显著相关。我们的数据表明，在无法获取大体新鲜组织时，通过乳腺钼靶引导下细针穿刺获取乳腺钼靶病变特异性新鲜细胞样本进行流式细胞术DNA分析，可评估乳腺钼靶检测到的DCIS病变。
