Zastawny T H, Kruszewski M, Olinski R
Department of Clinical Biochemistry, University School of Medical Sciences, Bydgoszcz, Poland.
Free Radic Biol Med. 1998 May;24(7-8):1250-5. doi: 10.1016/s0891-5849(97)00452-8.
Seven oxidized DNA bases were quantified, by gas GC/MS-SIM, in chromatin from gamma-rays and H2O2 treated mouse lymphoma L5178Y (LY) cells, inversely cross-sensitive to these agents. In H2O2 treated cells (2 mM, 1 h, 37 degrees C) we found more damage in LY-R cells than in LY-S cells. On the contrary, in gamma-rays (400 Gy) treated cells we found more damaged DNA bases in LY-S cells. The yield of damaged bases in control cells was similar in both cell lines, with the exception of 8OHAde and FapyGua that were found at a much higher level in LY-S cells. The yields of damaged bases were related to cellular sensitivity to damaging agent; this observation points to a relationship between DNA base damage induction, antioxidant defense system in the intracellular milieu and cell sensitivity.
通过气相色谱/质谱选择离子监测（GC/MS-SIM）对经γ射线和过氧化氢处理的小鼠淋巴瘤L5178Y（LY）细胞染色质中的七种氧化DNA碱基进行了定量分析，这些细胞对这些试剂呈反向交叉敏感。在过氧化氢处理的细胞（2 mM，1小时，37℃）中，我们发现LY-R细胞比LY-S细胞损伤更多。相反，在经γ射线（400 Gy）处理的细胞中，我们发现LY-S细胞中受损的DNA碱基更多。除了在LY-S细胞中发现的8-羟基腺嘌呤（8OHAde）和Fapy鸟嘌呤（FapyGua）水平高得多外，两种细胞系对照细胞中受损碱基的产量相似。受损碱基的产量与细胞对损伤剂的敏感性有关；这一观察结果表明DNA碱基损伤诱导、细胞内环境中的抗氧化防御系统与细胞敏感性之间存在关联。
