Vossbrinck C R, Andreadis T G, Debrunner-Vossbrinck B A
Connecticut Agricultural Experiment Station, New Haven 06504, USA.
J Eukaryot Microbiol. 1998 May-Jun;45(3):290-2. doi: 10.1111/j.1550-7408.1998.tb04538.x.
Small subunit rDNA sequences were obtained from field-collected Amblyospora connecticus (Microsporida: Amblyosporidae) spores isolated from the mosquito, Aedes cantator (Diptera: Culicidae), and from field collected spores isolated from the putative intermediate host, Acanthocyclops vernalis (Copepoda: Cyclopidae). The ribosomal DNA sequences of the spores isolated from the two hosts were identical. These findings corroborate previous laboratory transmission studies and validate the intermediary role of A. vernalis in the life cycle of this microsporidium. These data represent the first comparative sequence analysis of a microsporidium isolated from its definitive and intermediate hosts. The results demonstrate the effectiveness of using rDNA sequence data for screening potential intermediate hosts. Unlike laboratory transmission tests, which can take months or years to complete, this technique can be completed in days and can be performed on a single infected organism.
从小亚基核糖体DNA序列取自从野外采集的连接安布罗孢菌（微孢子虫纲：安布罗孢科）孢子，这些孢子是从蚊虫歌岸蚊（双翅目：蚊科）以及从野外采集的、来自假定中间宿主春季棘镖水蚤（桡足纲：镖水蚤科）的孢子中分离得到的。从这两种宿主分离得到的孢子的核糖体DNA序列是相同的。这些发现证实了先前的实验室传播研究，并验证了春季棘镖水蚤在这种微孢子虫生命周期中的中间宿主作用。这些数据代表了对从其终末宿主和中间宿主分离得到的微孢子虫的首次比较序列分析。结果证明了使用核糖体DNA序列数据筛选潜在中间宿主的有效性。与可能需要数月或数年才能完成的实验室传播试验不同，这项技术可以在数天内完成，并且可以在单个受感染生物体上进行。
