白细胞介素-13通过增强环氧化酶2（COX-2）基因表达，增加正常人多形核中性粒细胞中前列腺素E2（PGE2）的产生。

Interleukin-13 increases prostaglandin E2 (PGE2) production by normal human polymorphonuclear neutrophils by enhancing cyclooxygenase 2 (COX-2) gene expression.

作者信息

Yu C L, Huang M H, Kung Y Y, Tsai C Y, Tsai Y Y, Tsai S T, Huang D F, Sun K H, Han S H, Yu H S

机构信息

Department of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, Taiwan.

出版信息

Inflamm Res. 1998 Apr;47(4):167-73. doi: 10.1007/s000110050312.

DOI:10.1007/s000110050312

PMID:9628259

Abstract

OBJECTIVE

To investigate whether interleukin-13 (IL-13) can affect arachidonic acid metabolism and phagocytic activity of normal human polymorphonuclear neutrophils (PMN).

METHODS

Normal human PMN (1 x 10(6) cells/ml) were incubated with different concentrations of IL-13 (0.1-10 ng/ml) for a variety of times (30-120 min). Phagocytosis and intracellular cyclooxygenase-2 (COX-2) were detected by flow cytometry. The expression of COX-1 and COX-2 mRNA was detected by RT-PCR. The concentration of PGE2 in the PMN cultured supernatants was determined by EIA.

RESULTS

We found that IL-13 at an optimal concentration of 1 ng/ml significantly enhanced COX-2 gene expression and PGE2 production (121.57 +/- 22.17 pg/ml in IL-13 stimulation vs. 73.16 +/- 11.72 pg/ml in controls) by PMN. In addition, IL-13 stimulated PMN phagocytosis via increased complement receptor type 1 (CR1) and type 3 (CR3), but not IgG Fcgamma receptor type 3 (FcgammaRIII). The cytoplasmic neutral esterase activity of PMN was also enhanced by IL-13 stimulation for 24 h.

CONCLUSIONS

These results suggest that IL-13 can stimulate PMN and modulates the inflammatory reactions via the cyclooxygenase pathway.

摘要

目的

研究白细胞介素-13（IL-13）是否能影响正常人多形核中性粒细胞（PMN）的花生四烯酸代谢和吞噬活性。

方法

将正常人PMN（1×10⁶细胞/ml）与不同浓度的IL-13（0.1 - 10 ng/ml）孵育不同时间（30 - 120分钟）。通过流式细胞术检测吞噬作用和细胞内环氧合酶-2（COX-2）。通过RT-PCR检测COX-1和COX-2 mRNA的表达。通过酶免疫测定法测定PMN培养上清液中PGE2的浓度。

结果

我们发现，最佳浓度为1 ng/ml的IL-13可显著增强PMN的COX-2基因表达和PGE2产生（IL-13刺激组为121.57±22.17 pg/ml，对照组为73.16±11.72 pg/ml）。此外，IL-13通过增加1型补体受体（CR1）和3型补体受体（CR3）刺激PMN吞噬作用，但不通过3型IgG Fcγ受体（FcγRIII）。IL-13刺激24小时也可增强PMN的细胞质中性酯酶活性。