Fàbrega C, Güimil García R, Díaz A R, Eritja R
European Molecular Biology Laboratory, Heidelberg, Germany.
Biol Chem. 1998 Apr-May;379(4-5):527-33. doi: 10.1515/bchm.1998.379.4-5.527.
Oligonucleotides containing the photoreactive nucleosides 2-azido-2'-deoxyinosine and 8-azido-2'-deoxyadenosine have been prepared using protected 2-fluoro-2'-deoxyinosine and 8-bromo-2'-deoxyadenosine phosphoramidites. After the assembly of the oligonucleotides, the nucleoside derivatives are converted to the corresponding azido derivatives by treatment with lithium azide in dry DMF. Deprotection of oligonucleotides carrying these azidonucleosides is performed with concentrated ammonia at room temperature.
使用受保护的2-氟-2'-脱氧肌苷和8-溴-2'-脱氧腺苷亚磷酰胺制备了含有光反应性核苷2-叠氮基-2'-脱氧肌苷和8-叠氮基-2'-脱氧腺苷的寡核苷酸。在寡核苷酸组装完成后，通过在干燥的N,N-二甲基甲酰胺中用叠氮化锂处理，将核苷衍生物转化为相应的叠氮基衍生物。携带这些叠氮核苷的寡核苷酸在室温下用浓氨水进行脱保护。
