Maksimenko A V, Gottikh M B, Kubareva E A, Fedorova O A, Shabarova Z A
Belozersky Institute of Physical and Chemical Biology, Moscow State University, Russia.
Biol Chem. 1998 Apr-May;379(4-5):625-30.
Cyclic and polycyclic oligonucleotides were synthesized using chemical ligation. Two types of catenanes with one and several intertwinings were produced. The yield of these molecules depended on the ligation conditions and nucleotide sequence of the ligated oligonucleotide and its template. Structure of ligation products was investigated and confirmed using restriction endonuclease MvaI. Interaction of the synthesized molecules with restriction endonucleases SsoII, EcoRII and HindIII was also studied.
使用化学连接法合成了环状和多环寡核苷酸。制备了具有一个和几个缠绕结构的两种类型的连环体。这些分子的产率取决于连接条件、连接的寡核苷酸及其模板的核苷酸序列。使用限制性内切酶MvaI对连接产物的结构进行了研究和确认。还研究了合成分子与限制性内切酶SsoII、EcoRII和HindIII的相互作用。
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