Fujioka T, Jimi T, Hilliard B A, Ventura E S, Rostami A
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia 19104-4283, USA.
J Neuroimmunol. 1998 Apr 15;84(2):223-9. doi: 10.1016/s0165-5728(97)00252-x.
Autoreactive CD4+ T cells can transfer experimental allergic neuritis (EAN) to naive recipients. In order to further analyze the role of these T cells and their corresponding cytokines in EAN, we studied the expression of mRNA for IFN-gamma, IL-4, and IL-10 in the cauda equina of rats with EAN using a quantitative competitive reverse transcriptase PCR method. Nerves were studied on days 0 (pre-immunization), 10 (disease onset), 13 (clinical progression), 16 (disease peak), as well as 20, 24, and 34 post immunization (recovery). IFN-gamma messages increased at disease onset and peaked at day 13 p.i. IL-10 message remained at a very low level at disease onset and surged at day 16. Both messages were low in recovery stage. IL-4 message was undetectable at any time point. These data suggest a pro-inflammatory role of IFN-gamma and anti-inflammatory role of IL-10 in EAN lesions. It is also possible that a clonal switch from Th1 to Th2 occurs in EAN lesions during the disease course.
自身反应性CD4+ T细胞可将实验性变态反应性神经炎（EAN）传染给未致敏的受体。为了进一步分析这些T细胞及其相应细胞因子在EAN中的作用，我们采用定量竞争性逆转录酶PCR方法，研究了患EAN大鼠马尾中IFN-γ、IL-4和IL-10的mRNA表达。在免疫前第0天、发病第10天、临床进展期第13天、疾病高峰期第16天以及免疫后第20、24和34天（恢复期）对神经进行研究。IFN-γ信息在疾病发作时增加，并在免疫后第13天达到峰值。IL-10信息在疾病发作时保持在非常低的水平，并在第16天激增。在恢复期，这两种信息均较低。在任何时间点均未检测到IL-4信息。这些数据表明IFN-γ在EAN病变中具有促炎作用，而IL-10具有抗炎作用。在疾病过程中，EAN病变中也可能发生从Th1到Th2的克隆转换。
