Mesrobian H G
Division of Pediatric Urology, Children's Hospital and Medical College of Wisconsin, Milwaukee 53226, USA.
J Urol. 1998 Jul;160(1):146-9.
Previously the upper urinary tract anatomy has been characterized in Danforth mice spontaneous murine mutation. This mutation results in unilateral renal agenesis in 30% of the heterozygous offspring. Whether compensatory renal growth occurs in the remaining solitary kidney was assessed.
Heterozygous pairs of Danforth mice were mated. Of 99 kidneys recovered from adult offspring (8 weeks old) 41 were fixed in Bouin's solution, weighed, sectioned at 8 microm., stained and prepared for morphometry. An additional 6 kidneys recovered near term (gestational day 18) were processed in the same fashion. The kidneys from the mice without the mutation served as controls. Morphometric analyses were performed using computer software. The size and the distribution of glomeruli were studied.
The kidney-to-body weight ratio in the mice with 2 kidneys varied between 1.286 and 2.198 (mean 1.603 +/- 0.33). There were 9 solitary kidneys and their weight x 2/body weight ratio varied between 1.744 and 3.779 (mean 2.9583 +/- 0.724). The differences between these 2 groups were statistically significant (p = 0.003). In addition, the mean cross sectional areas in solitary kidney glomeruli was 13.2% greater than those in bilateral kidneys.
Previously described models of compensatory renal growth relied on surgically inducing varying degrees of renal ablation in intact animals with 2 kidneys, which introduced numerous variables in the study of a complex problem. The results demonstrate that the naturally occurring solitary kidneys of the Danforth mice undergo compensatory renal growth. Furthermore, this growth is initiated early in utero. The lack of anesthetic and surgical manipulation may make this an attractive, reliable and readily available model for the study of compensatory renal, including in utero, growth.
此前已对丹福斯小鼠（一种自发发生鼠类突变的小鼠）的上尿路解剖结构进行了表征。这种突变导致30%的杂合子后代出现单侧肾缺如。本研究评估了剩余的单个肾脏是否会发生代偿性肾脏生长。
将丹福斯小鼠的杂合子配对进行交配。从成年后代（8周龄）回收的99个肾脏中，41个固定于布安氏液中，称重，切成8微米厚的切片，染色并准备进行形态计量学分析。另外6个在接近足月（妊娠第18天）回收的肾脏也以同样方式处理。未发生突变的小鼠的肾脏作为对照。使用计算机软件进行形态计量学分析。研究了肾小球的大小和分布。
拥有两个肾脏的小鼠的肾重与体重之比在1.286至2.198之间（平均为1.603±0.33）。有9个单个肾脏，其重量×2/体重之比在1.744至3.779之间（平均为2.9583±0.724）。这两组之间的差异具有统计学意义（p = 0.003）。此外，单个肾脏肾小球的平均横截面积比双侧肾脏的肾小球大13.2%。
先前描述的代偿性肾脏生长模型依赖于在具有两个肾脏的完整动物中手术诱导不同程度的肾切除，这在研究一个复杂问题时引入了许多变量。结果表明，丹福斯小鼠自然出现的单个肾脏会发生代偿性肾脏生长。此外，这种生长在子宫内早期就开始了。缺乏麻醉和手术操作可能使这成为一个有吸引力、可靠且易于获得的模型，用于研究包括子宫内的代偿性肾脏生长。
