Augusteyn R C
National Vision Research Institute of Australia, Carlton, Victoria, Australia.
Exp Eye Res. 1998 May;66(5):669-74. doi: 10.1006/exer.1998.0468.
This work was undertaken to test the hypothesis that first exposure of the eye to light is responsible for the changes in lens protein expression patterns observed around the time of birth. The effect of light deprivation on lens properties was examined in Balb c mice which were bred, reared and maintained in complete darkness for up to 7 months. Data were collected on body weight, lens weight, lens protein contents and crystallin distributions. The data were compared with those obtained from age matched mice maintained in natural light/dark conditions. No significant differences were observed in body weight between animals maintained in the light and dark. However, animals kept in the dark had significantly smaller lenses. After 6 months in the dark, the lens represented 0.02% of body weight compared with 0.031% in the light reared animals (P < 0.001). Lens protein concentration, insoluble protein contents and crystallin synthesis patterns were indistinguishable for the two groups of animals. It is concluded that light stimulation of the eye is required for optimal lens growth but does not affect the production of specific crystallins.
眼睛首次接触光线是出生前后观察到的晶状体蛋白质表达模式变化的原因。在Balb c小鼠中研究了光线剥夺对晶状体特性的影响，这些小鼠在完全黑暗的环境中繁殖、饲养和维持长达7个月。收集了体重、晶状体重量、晶状体蛋白质含量和晶状体蛋白分布的数据。将这些数据与在自然光照/黑暗条件下饲养的年龄匹配小鼠的数据进行比较。在光照和黑暗环境中饲养的动物体重没有显著差异。然而，饲养在黑暗中的动物晶状体明显更小。在黑暗中饲养6个月后，晶状体占体重的0.02%，而在光照饲养的动物中为0.031%（P < 0.001）。两组动物的晶状体蛋白质浓度、不溶性蛋白质含量和晶状体蛋白合成模式没有区别。结论是，眼睛的光刺激是晶状体最佳生长所必需的，但不影响特定晶状体蛋白的产生。
