原弹性蛋白的放射免疫鉴定
Radioimmunological identification of tropoelastin.
作者信息
Christner P, Dixon M, Cywinski A, Rosenbloom J
出版信息
Biochem J. 1976 Aug 1;157(2):525-8. doi: 10.1042/bj1570525.
Abstract
Antiserum was prepared in sheep against insoluble elastin isolated from embryonic-chick aortae. In an indirect immunoprecipitation test, the antiserum reacted quantitatively with small amounts of radioactively labelled purified tropoelastin prepared from embryonic-chick aortae. The antiserum did not cross-react with chick procollagen, and the antiserum uas used to identify radioactively labelled tropoelastin secreted by chick aorta cells in suspension culture.
摘要
用从鸡胚主动脉分离出的不溶性弹性蛋白免疫绵羊制备抗血清。在间接免疫沉淀试验中，该抗血清能与少量由鸡胚主动脉制备的放射性标记纯化原弹性蛋白进行定量反应。该抗血清与鸡原胶原蛋白无交叉反应，并用此抗血清鉴定悬浮培养的鸡主动脉细胞分泌的放射性标记原弹性蛋白。
相似文献
1
Radioimmunological identification of tropoelastin.原弹性蛋白的放射免疫鉴定
Biochem J. 1976 Aug 1;157(2):525-8. doi: 10.1042/bj1570525.
2
Isolation of tropoelastin a from lathyritic chick aortae.从患骨生成障碍病的雏鸡主动脉中分离原弹性蛋白a
Biochem J. 1984 Jan 15;217(2):581-4. doi: 10.1042/bj2170581.
3
Kinetics of the incorporation of tropoelastin into elastic fibers in embryonic chick aorta.原弹性蛋白掺入胚胎期鸡主动脉弹性纤维的动力学
Connect Tissue Res. 1982;10(3-4):263-74. doi: 10.3109/03008208209008052.
4
Tropoelastin production and tropoelastin messenger RNA activity. Relationship to copper and elastin cross-linking in chick aorta.原弹性蛋白的产生及原弹性蛋白信使核糖核酸活性。与雏鸡主动脉中铜及弹性蛋白交联的关系。
Biochem J. 1986 Jul 1;237(1):17-23. doi: 10.1042/bj2370017.
5
Synthesis of soluble and insoluble elastins in cultures of chick aortic cells.鸡主动脉细胞培养物中可溶性和不溶性弹性蛋白的合成。
Lab Invest. 1988 Jun;58(6):667-73.
6
Fine mapping of tropoelastin-derived components in the aorta of developing chick embryo.
Histochem J. 1987 Dec;19(12):623-32. doi: 10.1007/BF01676168.
7
Multiple chick tropoelastin mRNAs.多种鸡原弹性蛋白信使核糖核酸
Biochem Biophys Res Commun. 1988 Aug 15;154(3):1054-60. doi: 10.1016/0006-291x(88)90247-1.
8
Identification of elastin in developing aortic tissue by immunological methods.
Connect Tissue Res. 1981;8(3-4):193-8. doi: 10.3109/03008208109152374.
9
Monoclonal antibodies as probes for differentiating tropoelastin a and b.用于区分原弹性蛋白a和b的单克隆抗体探针
Coll Relat Res. 1983 Nov;3(6):459-67. doi: 10.1016/s0174-173x(83)80026-0.
10
Biosynthesis of tropoelastin by elastic cartilage.
Connect Tissue Res. 1980;8(1):21-5. doi: 10.3109/03008208009152117.
引用本文的文献
1
Enhancement of humoral immune response against human lung elastin peptides.增强针对人肺弹性蛋白肽的体液免疫反应。
Experientia. 1980 May 15;36(5):606-7. doi: 10.1007/BF01965829.
2
Immunologic localization of elastin by electron microscopy.通过电子显微镜对弹性蛋白进行免疫定位
Am J Pathol. 1979 Aug;96(2):439-56.
本文引用的文献
1
Body size and tissue respiration.身体大小与组织呼吸
Biochim Biophys Acta. 1950 Jan;4(1-3):249-69. doi: 10.1016/0006-3002(50)90032-1.
2
Identification of a soluble intermediate during synthesis of elastin by embryonic chick aortae.
FEBS Lett. 1972 Mar 15;21(2):113-117. doi: 10.1016/0014-5793(72)80116-9.
3
Incorporation of labelled lysine into the desmosine cross-bridges in elastin.将标记的赖氨酸掺入弹性蛋白中的锁链素交联桥中。
Nature. 1966 Jan 22;209(5021):399-400. doi: 10.1038/209399b0.
4
Identification of a new crosslinking amino acid in elastin.
Biochem Biophys Res Commun. 1965 Dec 21;21(6):575-81. doi: 10.1016/0006-291x(65)90524-3.
5
The biosynthesis of elastin cross-links. The effect of copper deficiency and a lathyrogen.弹性蛋白交联的生物合成。铜缺乏和致畸形因素的影响。
J Biol Chem. 1965 Sep;240(9):3623-7.
6
The purification and partial characterization of a soluble elastin-like protein from copper-deficient porcine aorta.
Biochemistry. 1969 Jul;8(7):2940-5. doi: 10.1021/bi00835a037.
7
The cross-linking of collagen and elastin: enzymatic conversion of lysine in peptide linkage to alpha-aminoadipic-delta-semialdehyde (allysine) by an extract from bone.胶原蛋白与弹性蛋白的交联：骨提取物将肽键中的赖氨酸酶促转化为α-氨基己二酸-δ-半醛（赖氨醛）
Proc Natl Acad Sci U S A. 1968 Oct;61(2):708-16. doi: 10.1073/pnas.61.2.708.
8
Synthesis of soluble elastin by aortic medial cells in culture.
Biochem Biophys Res Commun. 1974 Jun 4;58(3):597-604. doi: 10.1016/s0006-291x(74)80461-4.
9
Molecular model for elastin structure and function.弹性蛋白结构与功能的分子模型。
Nature. 1973;246(5434):461-6. doi: 10.1038/246461a0.
10
Biosynthesis of soluble elastin by pig aortic tissue in vitro.
J Biol Chem. 1973 Dec 10;248(23):8157-61.
Zotero 插件