Cartwright R, Tambini C E, Simpson P J, Thacker J
Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxfordshire OX11 0RD, UK.
Nucleic Acids Res. 1998 Jul 1;26(13):3084-9. doi: 10.1093/nar/26.13.3084.
We recently identified a positional candidate for the XRCC2 DNA repair gene at human chromosome 7q36.1. We have now cloned the cDNA for this gene from both human and mouse and show that it is a highly conserved novel member of the recA / RAD51 recombination repair gene family. The cDNA is able to complement significantly the phenotype of a unique cell line, irs1 , which shows extreme sensitivity to DNA cross-linking agents and genetic instability. Thisphenotype is consistent with a role for the XRCC2 gene in recombination repair in somatic cells, suggesting that in addition to RAD51 , other members of this gene family have an important function in high fidelity repair processes in mammals. Despite this function, the XRCC2 gene transcript is expressed at a very low level in somatic tissue, but is elevated in mouse testis, suggesting an additional role in meiosis.
我们最近在人类染色体7q36.1上确定了XRCC2 DNA修复基因的一个位置候选基因。我们现已从人和小鼠中克隆出该基因的cDNA，并表明它是recA / RAD51重组修复基因家族中一个高度保守的新成员。该cDNA能够显著互补一种独特细胞系irs1的表型，该细胞系对DNA交联剂表现出极端敏感性且具有遗传不稳定性。这种表型与XRCC2基因在体细胞重组修复中的作用一致，这表明除了RAD51之外，该基因家族的其他成员在哺乳动物的高保真修复过程中也具有重要功能。尽管有此功能，但XRCC2基因转录本在体细胞组织中的表达水平非常低，而在小鼠睾丸中表达升高，提示其在减数分裂中还有额外作用。
