Okada Y, Okajima H
School of Health Sciences, Kyorin University, Tokyo, Japan.
Yakugaku Zasshi. 1998 Jun;118(6):226-30. doi: 10.1248/yakushi1947.118.6_226.
As the continuation of the work on the antioxidant properties of furan derivatives, we have studied hydroxyl radical (OH.) scavenging activity of dimethylfuran (DMF) and diphenylfuran (DPF), which are well-known as an effective scavenger of singlet oxygen, by an ESR spin trapping technique. The incubation mixture of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trapping agent with FE2+ and H202 in 50% acetonitrile aqueous solution gave 1:2:2:1 line signals characteristic of DMPO-OH spin adduct. The additions of furan derivatives to the incubation mixture decreased the intensity of the DMPO-OH spin adduct signal in a dose-dependent manner. The activities of furan derivatives were in the order of DMF > DPF > furan. The decrease in the intensity of the signals was not due to the inhibition of OH. generating system itself and the destruction of the spin adduct by furan derivatives. By comparison with the common OH. scavengers, DMF and DPF were found to scavenge OH. more effectively than dimethylsulfoxide and mannitol. These results indicate that DMF and DPF can act as a OH. scavenger as well as a singlet oxygen scavenger.
作为呋喃衍生物抗氧化性能研究工作的延续，我们通过电子自旋共振（ESR）自旋捕获技术，研究了作为单线态氧有效清除剂而闻名的二甲基呋喃（DMF）和二苯基呋喃（DPF）的羟自由基（OH·）清除活性。在50%乙腈水溶液中，自旋捕获剂5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）与Fe2+和H2O2的孵育混合物产生了DMPO-OH自旋加合物特有的1:2:2:1线信号。向孵育混合物中添加呋喃衍生物会以剂量依赖的方式降低DMPO-OH自旋加合物信号的强度。呋喃衍生物的活性顺序为DMF > DPF > 呋喃。信号强度的降低并非由于OH·产生系统本身受到抑制以及呋喃衍生物对自旋加合物的破坏。通过与常见的OH·清除剂比较，发现DMF和DPF清除OH·的效果比二甲基亚砜和甘露醇更有效。这些结果表明，DMF和DPF既可以作为OH·清除剂，也可以作为单线态氧清除剂。
