使用毛细管电泳法对比利时人群中四个短串联重复序列位点进行群体遗传学研究。

Population genetic study of four short tandem repeat loci in the Belgian population, using capillary electrophoresis.

作者信息

Van Hoofstat D E, Deforce D L, Millecamps R E, Brochez V M, Van Geldre E G, Vergauwe A, Sijs A, Van den Eeckhout E G

机构信息

Laboratory for Pharmaceutical Biotechnology, University of Ghent, Belgium.

出版信息

Electrophoresis. 1998 May;19(5):719-22. doi: 10.1002/elps.1150190520.

DOI:10.1002/elps.1150190520

PMID:9629905

Abstract

Allele frequencies of four short tandem repeat loci (HumCD4, HumTH01, HumD21S11 and HumSE33) were investigated in a sample of 395 unrelated Belgian individuals using multiplex polymerase chain reaction and capillary electrophoresis. Automated laser fluorescence was used to detect four fluorescent dyes, enabling the use of an internal standard within each lane. With this method rapid typing with high resolution was obtained and the different alleles were grouped on a statistical base. All loci meet Hardy-Weinberg expectations. The allelic frequency data, together with the constructed allelic ladder, can be used in paternity testing and personal identification in the medical and forensic sciences.

摘要

使用多重聚合酶链反应和毛细管电泳技术，对395名无亲缘关系的比利时个体样本中的四个短串联重复序列位点（HumCD4、HumTH01、HumD21S11和HumSE33）的等位基因频率进行了研究。使用自动激光荧光检测四种荧光染料，从而能够在每个泳道内使用内标。通过这种方法获得了高分辨率的快速分型，并在统计学基础上对不同等位基因进行了分组。所有位点均符合哈迪-温伯格平衡预期。等位基因频率数据以及构建的等位基因阶梯可用于医学和法医学中的亲子鉴定和个人身份识别。