Ricciardone M D, Lins A M, Schumm J W, Holland M M
Armed Forces Institute of Pathology, Rockville, MD, USA.
Biotechniques. 1997 Oct;23(4):742-7.
Short tandem repeat (STR) loci are ideal markers for personal identification and for genomic mapping. Two fluorescent multiplex systems, each designed for simultaneous PCR amplification of four polymorphic STR loci (HUMCSF1PO, HUMTPOX, HUMTH01 and HUMVWFA31, and HUMF13A01, HUMFESFPS, HUMBFXIII and HUMLIPOL), were evaluated on three laser fluorescence detection instruments. Concordant DNA typing results were obtained with all three detection methods. These fluorescent multiplex STR systems offer an accurate, reproducible and versatile method of DNA profiling that is well-suited for forensic identity testing and other genetic analyses.
短串联重复序列（STR）位点是用于个人识别和基因组作图的理想标记。两种荧光多重系统，每种都设计用于同时PCR扩增四个多态性STR位点（HUMCSF1PO、HUMTPOX、HUMTH01和HUMVWFA31，以及HUMF13A01、HUMFESFPS、HUMBFXIII和HUMLIPOL），在三种激光荧光检测仪器上进行了评估。所有三种检测方法均获得了一致的DNA分型结果。这些荧光多重STR系统提供了一种准确、可重复且通用的DNA分析方法，非常适合法医身份鉴定测试和其他遗传分析。
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