Yamada G, Nakamura S, Haraguchi R, Terai K, Nomura S, Kitamura Y, Minami T, Yamada M O, Suzuki S, Izumi H, Nagata R
Cellular and Developmental Biology Division, Kurume University Research Center for Innovative Cancer Therapy, Fukuoka, Japan.
Biol Trace Elem Res. 1998 Apr-May;62(1-2):75-82. doi: 10.1007/BF02820023.
It has been recognized that bone trace element composition analysis provides clues when analyzing bone-related physiological conditions. Increasing numbers of bone-related genetic diseases have been identified recently. In this study, we have analyzed bone trace element composition in a genetic mutant animal model. Mutations in the mouse microphthalmia (mi) gene affect the development of a number of cell types, including melanocytes, mast cells, and osteoclasts. Previous studies have shown that different alleles of the mi locus show osteopetrosis. In order to gain insights into the effects of a particular genetic defect on bone trace element composition and bone structure, we performed bone trace element composition analysis using inductively coupled plasma atomic emissions spectrometry (ICP-AES). Marked changes in bone trace element levels were found in vertebrate bones of mi mutant mice. The implications and possible applications of bone trace element analysis will be discussed in this article.
