Sasaki Y F, Saga A, Yoshida K, Su Y Q, Ohta T, Matsusaka N, Tsuda S
Laboratory of Genotoxicity, Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology, Tamonoki Uwanotai 16-1, Hachinohe, Aomori 039-11, Japan.
Mutat Res. 1998 May 11;414(1-3):9-14. doi: 10.1016/s1383-5718(98)00033-3.
The in vivo genotoxicity of five heterocyclic amines-Trp-P-2 (13 mg/kg), IQ (13 mg/kg), MeIQ (13 mg/kg), MeIQx (13 mg/kg), and PhIP (40 mg/kg)-in the mucosa of gastrointestinal and urinary tract organs (stomach, duodenum, jejunum, ileum, colon, and bladder) was studied by the alkaline single cell gel electrophoresis (SCG) (Comet) assay. Male CD-1 mice were sacrificed 1, 3, and 8 h after intraperitoneal injection. All the heterocyclic amines studied yielded statistically significant DNA damage in the colon but not the small intestine (duodenum, jejunum, and ileum) or urinary bladder. In this study, five heterocyclic amines were injected intraperitoneally to avoid the consequences of ingestion. Thus, the extensive damage to colon DNA was concluded to be due, at least in part, to a systemic effect.
通过碱性单细胞凝胶电泳（SCG）（彗星）试验，研究了五种杂环胺——Trp-P-2（13毫克/千克）、IQ（13毫克/千克）、MeIQ（13毫克/千克）、MeIQx（13毫克/千克）和PhIP（40毫克/千克）——对胃肠道和泌尿系统器官（胃、十二指肠、空肠、回肠、结肠和膀胱）黏膜的体内遗传毒性。雄性CD-1小鼠在腹腔注射后1、3和8小时被处死。所有研究的杂环胺在结肠中均产生了具有统计学意义的DNA损伤，但在小肠（十二指肠、空肠和回肠）或膀胱中未产生。在本研究中，通过腹腔注射五种杂环胺以避免摄入带来的影响。因此，得出结论，结肠DNA的广泛损伤至少部分归因于全身效应。
