Lu L, Komada M, Kitamura N
Institute for Liver Research, Kansai Medical University, Moriguchi, Osaka 570, Japan.
Gene. 1998 Jun 15;213(1-2):125-32. doi: 10.1016/s0378-1119(98)00184-x.
Hrs is a 115kDa zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. We previously purified the protein from a mouse cell line and cloned its cDNA. In the present study, we cloned a human Hrs cDNA from a human placenta cDNA library by cross-hybridization, using the mouse cDNA as a probe, and determined its nucleotide sequence. The human Hrs cDNA encoded a 777-amino-acid protein whose sequence was 93% identical to that of mouse Hrs. Northern blot analysis showed that the Hrs mRNA was about 3.0kb long and was expressed in all the human adult and fetal tissues tested. In addition, we showed by genomic Southern blot analysis that the human Hrs gene was a single-copy gene with a size of about 20kb. Furthermore, the human Hrs gene was mapped to chromosome 17 by Southern blotting of genomic DNAs from human/rodent somatic cell hybrids.
Hrs是一种115kDa的锌指蛋白，在受到各种生长因子刺激的细胞中会迅速发生酪氨酸磷酸化。我们之前从小鼠细胞系中纯化了该蛋白并克隆了其cDNA。在本研究中，我们以小鼠cDNA为探针，通过杂交从人胎盘cDNA文库中克隆了人Hrs cDNA，并测定了其核苷酸序列。人Hrs cDNA编码一个777个氨基酸的蛋白质，其序列与小鼠Hrs的序列有93%的同一性。Northern印迹分析表明，Hrs mRNA约3.0kb长，在所检测的所有成人和胎儿人体组织中均有表达。此外，我们通过基因组Southern印迹分析表明，人Hrs基因是一个单拷贝基因，大小约为20kb。此外，通过对人/啮齿动物体细胞杂种的基因组DNA进行Southern印迹分析，将人Hrs基因定位到了17号染色体上。
