Arias W M, Pettersson H, Pettersson G
Department of Biochemistry, Chemical Center, University of Lund, Lund, Sweden.
Biochim Biophys Acta. 1998 Jun 11;1385(1):149-56. doi: 10.1016/s0167-4838(98)00035-1.
Steady-state and transient-state kinetic experiments have been performed to test the proposal that there is a direct (channelled) transfer of NADH from one dehydrogenase to another if the two enzymes exhibit distinct chiral coenzyme specificity (A-side vs. B-side). The results lend no support to this proposal, but are fully consistent with a free-diffusion mechanism of NADH transfer irrespective of the chiral specificity of the enzymes.
进行了稳态和瞬态动力学实验，以检验以下提议：如果两种酶表现出不同的手性辅酶特异性（A面与B面），则烟酰胺腺嘌呤二核苷酸（NADH）会从一种脱氢酶直接（通道化）转移到另一种脱氢酶。结果不支持这一提议，但完全符合NADH转移的自由扩散机制，而与酶的手性特异性无关。
