Loidl-Stahlhofen A, Kaufmann S, Braunschweig T, Bayerl T M
Universität Würzburg, Physikalisches Institut EP-5, Germany.
Nat Biotechnol. 1996 Aug;14(8):999-1002. doi: 10.1038/nbt0896-999.
A new concept of charge-selective bioseparation with certain advantages over the established ion-exchange technique is reported. The procedure is based on the temperature-controlled creation and dispersion of domain structures in single phospholipid bilayers by means of the phase transition between the gel phase and the fluid phase of the bilayer. The bilayers are presented on a solid support of silica gel. Rather than altering the ionic strength of the buffer, protein elution is accomplished by a change of the chromatography column temperature. The application of temperature gradients improves the protein selectivity of phase transition chromatography.
报道了一种电荷选择性生物分离的新概念，它相对于已有的离子交换技术具有某些优势。该方法基于通过双层磷脂的凝胶相和流体相之间的相变，在单个磷脂双层中进行温度控制的域结构的创建和分散。双层磷脂存在于硅胶固体支持物上。蛋白质洗脱不是通过改变缓冲液的离子强度来实现，而是通过改变色谱柱温度来完成。温度梯度的应用提高了相变色谱的蛋白质选择性。
